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Purification, Characterization, and Biocatalytic and Antibiofilm Activity of a Novel Dextranase from Talaromyces sp.
المؤلفون المشاركون
Hassan, Ramadan
Adel El-Sokkary, Mohamed M.
Ebaya, Mahasen Mohamed Ahmed
El-Mowafy, Mohammed
المصدر
International Journal of Microbiology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-11
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Dextranase is a useful enzyme that catalyzes the degradation of dextran to low-molecular-weight fractions, which have many critical commercial and clinical applications.
Endophytic fungi represent a source of both high heat-stable and pH-stable enzymes.
In this study, from Delonix regia bark by plate assay, out of 12 isolated fungal strains, hyaline zones were detected in only one strain.
By using the standard ITS rDNA sequencing analysis, the isolated strain was identified as Talaromyces sp.
In the case of carbon source, in a medium containing 1% dextran T2000 as the sole carbon source, the maximum dextranase activity reached approximately 120 U/ml after incubation of 2 days where the optimum pH was 7.4.
Peptone addition to the production medium as a sole nitrogen source was accompanied by a significant increase in the dextranase production.
Similarly, some metal ions, such as Fe2+ and Zn2+, increased significantly enzyme production.
However, there was no significant difference resulting from the addition of Cu2+.
The crude dextranase was purified by ammonium sulfate fractionation, followed by Sephadex G100 chromatography with 28-fold purification.
The produced dextranase was 45 kDa with an optimum activity at 37°C and a pH of 7.
Moreover, the presence of MgSO4, FeSO4, and NH4SO4 increased the purified dextranase activity; however, SDS and EDTA decreased it.
Interestingly, the produced dextranase expressed remarkable pH stability, temperature stability, and biofilm inhibition activity, reducing old-established biofilm by 86% and biofilm formation by 6%.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ebaya, Mahasen Mohamed Ahmed& El-Mowafy, Mohammed& Adel El-Sokkary, Mohamed M.& Hassan, Ramadan. 2020. Purification, Characterization, and Biocatalytic and Antibiofilm Activity of a Novel Dextranase from Talaromyces sp.. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172547
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ebaya, Mahasen Mohamed Ahmed…[et al.]. Purification, Characterization, and Biocatalytic and Antibiofilm Activity of a Novel Dextranase from Talaromyces sp.. International Journal of Microbiology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1172547
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ebaya, Mahasen Mohamed Ahmed& El-Mowafy, Mohammed& Adel El-Sokkary, Mohamed M.& Hassan, Ramadan. Purification, Characterization, and Biocatalytic and Antibiofilm Activity of a Novel Dextranase from Talaromyces sp.. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172547
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172547
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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