The Effect of Citalopram on Genome-Wide DNA Methylation of Human Cells
المؤلفون المشاركون
Varma, Sudhir
Kanherkar, Riya R.
Csoka, Antonei B.
Getachew, Bruk
Ben-Sheetrit, Joseph
Heinbockel, Thomas
Tizabi, Yousef
المصدر
International Journal of Genomics
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-07-25
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Commonly used pharmaceutical drugs might alter the epigenetic state of cells, leading to varying degrees of long-term repercussions to human health.
To test this hypothesis, we cultured HEK-293 cells in the presence of 50 μM citalopram, a common antidepressant, for 30 days and performed whole-genome DNA methylation analysis using the NimbleGen Human DNA Methylation 3x720K Promoter Plus CpG Island Array.
A total of 626 gene promoters, out of a total of 25,437 queried genes on the array (2.46%), showed significant differential methylation (p<0.01); among these, 272 were hypomethylated and 354 were hypermethylated in treated versus control.
Using Ingenuity Pathway Analysis, we found that the chief gene networks and signaling pathways that are differentially regulated include those involved in nervous system development and function and cellular growth and proliferation.
Genes implicated in depression, as well as genetic networks involving nucleic acid metabolism, small molecule biochemistry, and cell cycle regulation were significantly modified.
Involvement of upstream regulators such as BDNF, FSH, and NFκB was predicted based on differential methylation of their downstream targets.
The study validates our hypothesis that pharmaceutical drugs can have off-target epigenetic effects and reveals affected networks and pathways.
We view this study as a first step towards understanding the long-term epigenetic consequences of prescription drugs on human health.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kanherkar, Riya R.& Getachew, Bruk& Ben-Sheetrit, Joseph& Varma, Sudhir& Heinbockel, Thomas& Tizabi, Yousef…[et al.]. 2018. The Effect of Citalopram on Genome-Wide DNA Methylation of Human Cells. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1172895
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kanherkar, Riya R.…[et al.]. The Effect of Citalopram on Genome-Wide DNA Methylation of Human Cells. International Journal of Genomics No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1172895
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kanherkar, Riya R.& Getachew, Bruk& Ben-Sheetrit, Joseph& Varma, Sudhir& Heinbockel, Thomas& Tizabi, Yousef…[et al.]. The Effect of Citalopram on Genome-Wide DNA Methylation of Human Cells. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1172895
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1172895
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر