Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia
المؤلفون المشاركون
Alqasim, Abdulaziz
Abu Jaffal, Ahmad
Alyousef, Abdullah A.
المصدر
International Journal of Microbiology
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-16
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The prevalence of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-producing E.
coli) has recently increased worldwide.
This study aims at determining the antimicrobial susceptibility patterns of a collection of clinical E.
coli urine isolates and evaluating the ESBL carriage of these isolates at phenotypic and genotypic levels.
A total of 100 E.
coli urine isolates were collected at a tertiary healthcare centre in Riyadh from January 2018 to March 2018.
Antimicrobial susceptibility testing was carried out for all isolates.
ESBL production was characterized at phenotypic and genotypic levels using double-disc synergy test and polymerase chain reaction, respectively.
Detection of different ESBL variants was performed using DNA sequencing.
Of 100 E.
coli isolates, 67 were associated with multidrug resistance (MDR) phenotype.
All isolates showed variable resistance levels to all antibiotics used here expect to imipenem, where they were all imipenem-sensitive.
33 out of 100 E.
coli isolates were positive for ESBLs by phenotypic and genotypic methods.
Among all ESBL-positive E.
coli isolates, the CTX-M was the most prevalent ESBL type (31/33 isolates; 93.94%).
CTX-M-15 variant was detected in all isolates associated with CTX-M carriage.
Multiple ESBL gene carriage was detected in 15/33 isolates (45.45%), where 11 (33.33%) isolates produced two different ESBL types while 4 isolates (12.12%) associated with carrying three different ESBL types.
Our study documented the high antimicrobial resistance of ESBL-producing E.
coli to many front-line antibiotics currently used to treat UTI patients, and this implies the need to continuously revise the local guidelines used for optimal empirical therapy for UTI patients.
It also showed the high prevalence of ESBL carriage in E.
coli urine isolates, with CTX-M-15 being the most predominant CTX-M variant.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alqasim, Abdulaziz& Abu Jaffal, Ahmad& Alyousef, Abdullah A.. 2018. Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia. International Journal of Microbiology،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1173305
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alqasim, Abdulaziz…[et al.]. Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia. International Journal of Microbiology No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1173305
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alqasim, Abdulaziz& Abu Jaffal, Ahmad& Alyousef, Abdullah A.. Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia. International Journal of Microbiology. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1173305
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1173305
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر