Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen
المؤلفون المشاركون
Zhang, Ling
Yang, Xiaocui
Xiao, Kaijun
Lu, Yuyi
Li, Chunhai
Zhang, Zijin
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-09-26
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
The structure of an alkaline protease from Bacillus subtilis used by a tilapia collagen peptide manufacturer was analyzed, and the technology of the enzyme immobilized by sulfonated polystyrene (SPS) nanoparticles was studied.
The particle size distribution, SEM, EDS, TEM, and FT-IR spectroscopy of the carrier before and after immobilization were analyzed.
The results showed that the molecular weight of the purified enzyme protein was 31.0 kDa.
The amino acid sequence with a consistency of 64.04% and one three-dimensional structure simulation diagram of the purified enzyme protein were obtained by LC-MS-MS, which suggested that the protein might belong to subtilisin.
The optimal immobilization conditions were as follows: the volume ratio of the immobilization carrier to the enzyme was 3 : 50 (mL : mL), the immobilized temperature was 25°C, and the system pH was 4.5.
Under this condition, the immobilization ratio of collagenase was 73.48%, the specific activity was 274.05 U/μg, and the specific activity of the immobilized enzyme was about 53.74% that of the free enzyme.
The average particle size of SPS nanospheres was 155.1 nm.
The characterization results of SEM, EDS, TEM, and FT-IR spectroscopy showed that the collagenase was successfully immobilized onto SPS nanospheres.
The experimental results also showed that the collagenase could be immobilized effectively under the optimal conditions by using SPS nanospheres, and the operation process was simple, feasible, and of low cost with good prospect of industrial application.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Ling& Yang, Xiaocui& Xiao, Kaijun& Lu, Yuyi& Li, Chunhai& Zhang, Zijin. 2019. Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen. Journal of Food Quality،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1174493
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Ling…[et al.]. Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen. Journal of Food Quality No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1174493
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Ling& Yang, Xiaocui& Xiao, Kaijun& Lu, Yuyi& Li, Chunhai& Zhang, Zijin. Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen. Journal of Food Quality. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1174493
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1174493
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر