Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLCESI-Q-TOF MS and Their Protective Effects on PC12 Cells
المؤلفون المشاركون
Miao, Jianhua
Hao, Erwei
Qin, Jianfeng
Wei, Wei
Xie, Yan
Pan, Xianglong
Wu, Hangxuan
Xie, Jinling
Fan, Xiaosu
Du, Zhengcai
Hou, Xiaotao
Deng, Jiagang
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-09
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Yizhi Granule (YZG) is a health food containing six traditional Chinese medicines (TCMs).
It improves memory barriers in rat experiments.
Here, we describe the first fast and sensitive ultraperformance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF MS) method for analyzing YZG in plasma.
We used this technique for studies in cynomolgus monkey plasma.
By comparing retention time, MS, and MS/MS data of reference compounds, 70 compounds were detected in YZG.
Of these, 63 were identified including 60 saponins, 2 flavones, and 1 methyl ester.
There were 33 saponins, 1 flavone, and 1 methyl ester in the plasma.
Next, to study the therapeutic properties of YZG, the neuroprotective effect of some of the absorbed components was evaluated using PC12 cell damage caused by the Aβ25–35 model.
The results showed that 9 compounds protect PC12 cells from Aβ25–35 with cell viability (%) of 111.00 ± 8.12 (G-Rb1), 102.20 ± 4.22 (G-Rb2), 100.34 ± 6.47 (G-Rd), 102.83 ± 2.10 (G-Re), 101.68 ± 7.64 (NG-Fa), 101.19 ± 7.83 (NG-R1), 102.53 ± 0.55 (NG-R2), 106.88 ± 4.95 (gypenoside A), and 103.95 ± 4.11 (gypenoside XLIX), respectively, versus the control group (87.51 ± 6.59).
These results can reveal the real pharmacodynamic basis of YZG and provide a theoretical basis for subsequent studies.
It can also provide some references for the research of Alzheimer’s disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hao, Erwei& Qin, Jianfeng& Wei, Wei& Miao, Jianhua& Xie, Yan& Pan, Xianglong…[et al.]. 2020. Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLCESI-Q-TOF MS and Their Protective Effects on PC12 Cells. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1174758
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hao, Erwei…[et al.]. Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLCESI-Q-TOF MS and Their Protective Effects on PC12 Cells. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1174758
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hao, Erwei& Qin, Jianfeng& Wei, Wei& Miao, Jianhua& Xie, Yan& Pan, Xianglong…[et al.]. Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLCESI-Q-TOF MS and Their Protective Effects on PC12 Cells. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1174758
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1174758
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر