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Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA
المؤلفون المشاركون
Huang, Jian
Yang, Lu
Ma, Qingdong
Chen, Xiaoxiang
Zhang, Ke
Yao, Dahong
Wang, Hangyu
Bulemasi, Santai
Wang, Jinhui
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The quality control of Saussurea involucrata has been greatly improved by macroscopic and microscopic identification and chemical profiling described in Chinese Pharmacopoeia since 2005.
However, these methods have their own limitations, e.g., their dependence on personal experience and expertise, and it is a huge challenge to identify closely related species that share similar or identical morphological characteristics and chemical profiles.
A novel and generally accepted identification strategy is urgently needed as a complement to regulations for protecting the public health interests.
In this work, a comprehensive chromatographic fingerprint method was developed and tested on 43 samples from four haplotypes of S.
involucrata according to DNA barcoding.
Three common patterns consisting of 20, 14, and 7 common peaks were generated by frequency filters of median, upper quartile, and 100%, respectively.
Based on two formerly screened patterns, S.
involucrata can be effectively identified from its five easily confused snow lotus species, including the most closely related plant (S.
orgaadayi) in the orthogonal partial least-squares discriminant analysis (OPLS-DA) models.
The model is supported by good R and Q coefficients.
In addition, different haplotypes of S.
involucrata can be discriminated in the OPLS-DA model using the 20 common peaks.
Among them, peaks 9, 11, 16 (zaluzanin C), and 18 (dehydrocostus lactone) have been identified as fingerprint markers of S.
involucrata via S-plots and VIP values (>1).
Additionally, peaks 19 and 20 were identified as linolenic acid and linoleic acid with anti-inflammatory activity, and they were isolated from the herb for the first time.
Collectively, the chromatographic fingerprint of S.
involucrata can be an effective and integrated method for the identification of authentic herbs from adulterant species or related plants, and discrimination of its different haplotypes provides an objective and reliable tool for quality control.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ma, Qingdong& Chen, Xiaoxiang& Zhang, Ke& Yao, Dahong& Yang, Lu& Wang, Hangyu…[et al.]. 2020. Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1174848
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ma, Qingdong…[et al.]. Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1174848
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ma, Qingdong& Chen, Xiaoxiang& Zhang, Ke& Yao, Dahong& Yang, Lu& Wang, Hangyu…[et al.]. Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1174848
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1174848
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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