Expression of USP18 and IL2RA Is Increased in Individuals Receiving Latent Tuberculosis Treatment with Isoniazid
المؤلفون المشاركون
Wilkinson, Robert J.
de Oyarzabal, Eleane
García-García, Lourdes
Ferreyra-Reyes, Leticia
Orozco, Lorena
Herrera, María Teresa
Carranza, Claudia
Sada, Eduardo
Juárez, Esmeralda
Ponce-de-León, Alfredo
Sifuentes-Osornio, José
Rangel-Escareño, Claudia
Torres, Martha
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-06
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background.
The treatment of latent tuberculosis infection (LTBI) in individuals at risk of reactivation is essential for tuberculosis control.
However, blood biomarkers associated with LTBI treatment have not been identified.
Methods.
Blood samples from tuberculin skin test (TST) reactive individuals were collected before and after one and six months of isoniazid (INH) therapy.
Peripheral mononuclear cells (PBMC) were isolated, and an in-house interferon-γ release assay (IGRA) was performed.
Expression of chemokine ligand 4 (CCL4), chemokine ligand 10 (CXCL10), chemokine ligand 11 (CXCL11), interferon alpha (IFNA), radical S-adenosyl methionine domain-containing 2 (RSAD2), ubiquitin-specific peptidase 18 (USP18), interferon-induced protein 44 (IFI44), interferon-induced protein 44 like (IFI44L), interferon-induced protein tetratricopeptide repeats 1(IFIT1), and interleukin 2 receptor subunit alpha (IL2RA) mRNA levels were assessed by qPCR before, during, and after INH treatment.
Results.
We observed significantly lower relative abundances of USP18, IFI44L, IFNA, and IL2RA transcripts in PBMC from IGRA-positive individuals compared to levels in IGRA-negative individuals before INH therapy.
Also, relative abundance of CXCL11 was significantly lower in IGRA-positive than in IGRA-negative individuals before and after one month of INH therapy.
However, the relative abundance of CCL4, CXCL10, and CXCL11 mRNA was significantly decreased and that of IL2RA and USP18 significantly increased after INH therapy, regardless of the IGRA result.
Our results show that USP18, IFI44L, IFIT1, and IL2RA relative abundances increased significantly, meanwhile the relative abundance of CCL4, CXCL11, and IFNA decreased significantly after six months of INH therapy in TST-positive individuals.
Conclusions.
Changes in the profiles of USP18, IL2RA, IFNA, CCL4, and CXCL11 expressions during INH treatment in TST-positive individuals, regardless of IGRA status, are potential tools for monitoring latent tuberculosis treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
de Oyarzabal, Eleane& García-García, Lourdes& Rangel-Escareño, Claudia& Ferreyra-Reyes, Leticia& Orozco, Lorena& Herrera, María Teresa…[et al.]. 2019. Expression of USP18 and IL2RA Is Increased in Individuals Receiving Latent Tuberculosis Treatment with Isoniazid. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1175660
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
de Oyarzabal, Eleane…[et al.]. Expression of USP18 and IL2RA Is Increased in Individuals Receiving Latent Tuberculosis Treatment with Isoniazid. Journal of Immunology Research No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1175660
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
de Oyarzabal, Eleane& García-García, Lourdes& Rangel-Escareño, Claudia& Ferreyra-Reyes, Leticia& Orozco, Lorena& Herrera, María Teresa…[et al.]. Expression of USP18 and IL2RA Is Increased in Individuals Receiving Latent Tuberculosis Treatment with Isoniazid. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1175660
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1175660
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر