43 kDa Glycoprotein (gp43) from Paracoccidioides brasiliensis Induced IL-17A and PGE2 Production by Human Polymorphonuclear Neutrophils: Involvement of TLR2 and TLR4
المؤلفون المشاركون
Dias-Melicio, Luciane Alarcão
Soares, Angela Maria Victoriano Campos
Della Coletta, Amanda Manoel
Gardizani, Taiane Priscila
Romagnoli, Graziela Gorete
Puccia, Rosana
Serezani, Ana Paula Moreira
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-26
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The glycoprotein gp43 is the major antigenic/diagnostic component of Paracoccidioides brasiliensis, one of the etiologic agents of paracoccidioidomycosis (PCM).
Gp43 has protective roles in mice, but due to adhesive properties, this glycoprotein has also been associated with immune evasion mechanisms.
The present study evaluated gp43 interaction in vitro with Toll-like receptors 2 and 4 (TLR2 and TLR4) present in polymorphonuclear neutrophils (PMNs) from healthy human individuals and the consequent modulation of the immune response through the expression and release of cytokines and eicosanoids.
PMNs were incubated in the absence or presence of monoclonal antibodies anti-TLR2 and anti-TLR4 (individually or in combination) before gp43 stimulation.
Then, PMNs were analyzed for the expression of both surface receptors and the detection of intracytoplasmic IL-17A and IL-4 using flow cytometry, while the production of PGE2, LTB4, IL-6, IL-10, IL-12, IFN-γ, and TNF-α was evaluated in the supernatants by enzyme-linked immunosorbent assay (ELISA).
Our results showed that gp43 increased TLR2 and TLR4 expression by PMNs and induced PGE2 and IL-17A via TLR4 and TLR2, respectively.
Thus, our data suggest that gp43 from P.
brasiliensis might modulate host susceptibility to the fungal infection by affecting PGE2 and IL-17A production.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gardizani, Taiane Priscila& Della Coletta, Amanda Manoel& Romagnoli, Graziela Gorete& Puccia, Rosana& Serezani, Ana Paula Moreira& Soares, Angela Maria Victoriano Campos…[et al.]. 2019. 43 kDa Glycoprotein (gp43) from Paracoccidioides brasiliensis Induced IL-17A and PGE2 Production by Human Polymorphonuclear Neutrophils: Involvement of TLR2 and TLR4. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1175735
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gardizani, Taiane Priscila…[et al.]. 43 kDa Glycoprotein (gp43) from Paracoccidioides brasiliensis Induced IL-17A and PGE2 Production by Human Polymorphonuclear Neutrophils: Involvement of TLR2 and TLR4. Journal of Immunology Research No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1175735
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gardizani, Taiane Priscila& Della Coletta, Amanda Manoel& Romagnoli, Graziela Gorete& Puccia, Rosana& Serezani, Ana Paula Moreira& Soares, Angela Maria Victoriano Campos…[et al.]. 43 kDa Glycoprotein (gp43) from Paracoccidioides brasiliensis Induced IL-17A and PGE2 Production by Human Polymorphonuclear Neutrophils: Involvement of TLR2 and TLR4. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1175735
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1175735
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر