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Insight into Neutrophil Extracellular Traps through Systematic Evaluation of Citrullination and Peptidylarginine Deiminases
المؤلفون المشاركون
Holmes, Caitlyn L.
Shim, Daeun
Kernien, John
Johnson, Chad J.
Nett, Jeniel E.
Shelef, Miriam A.
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-12
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
In rheumatoid arthritis, an autoimmune inflammatory arthritis, citrullinated proteins are targeted by autoantibodies and thus thought to drive disease.
Neutrophil extracellular traps (NETs) are a source of citrullinated proteins and are increased in rheumatoid arthritis and therefore also implicated in disease pathogenesis.
However, not all NETs are citrullinated.
One theory aiming to clarify the intersection of citrullination, NETs, and rheumatoid arthritis suggests that specific stimuli induce different types of NETs defined by citrullination status.
However, most studies do not evaluate uncitrullinated NETs, only citrullinated or total NETs.
Further, the requirement for peptidylarginine deiminase (PAD) 2 and 4, two important citrullinating enzymes in neutrophils and rheumatoid arthritis, in the formation of different NETs has not been clearly defined.
To determine if specific stimulants induce citrullinated or uncitrullinated NETs and if those structures require PAD2 or PAD4, human and murine neutrophils, including from PAD4-/- and PAD2-/- mice, were stimulated in vitro and NETs imaged and quantified.
In humans, phorbol myristate acetate (PMA), ionomycin, monosodium urate (MSU), and Candida albicans induced NETs with MSU and C.
albicans inducing primarily citrullinated, PMA primarily uncitrullinated, and ionomycin a mix of NETs.
Only ionomycin and C.
albicans were strong inducers of NETs in mice with ionomycin-induced NETs mostly citrullinated and C.
albicans-induced NETs a mix of citrullinated and uncitrullinated.
Interestingly, no stimulus induced exclusively citrullinated or uncitrullinated NETs.
Further, PAD4 was required for citrullinated NETs only, whereas PAD2 was not required for either NET in mice.
Therefore, specific stimuli induce varying proportions of both citrullinated and uncitrullinated NETs with different requirements for PAD4.
These findings highlight the complexity of NET formation and the need to further define the mechanisms by which different NETs form and their implications for autoimmune disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Holmes, Caitlyn L.& Shim, Daeun& Kernien, John& Johnson, Chad J.& Nett, Jeniel E.& Shelef, Miriam A.. 2019. Insight into Neutrophil Extracellular Traps through Systematic Evaluation of Citrullination and Peptidylarginine Deiminases. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1175823
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Holmes, Caitlyn L.…[et al.]. Insight into Neutrophil Extracellular Traps through Systematic Evaluation of Citrullination and Peptidylarginine Deiminases. Journal of Immunology Research No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1175823
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Holmes, Caitlyn L.& Shim, Daeun& Kernien, John& Johnson, Chad J.& Nett, Jeniel E.& Shelef, Miriam A.. Insight into Neutrophil Extracellular Traps through Systematic Evaluation of Citrullination and Peptidylarginine Deiminases. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1175823
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1175823
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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