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Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoate in Bulk and Tablets
المؤلفون المشاركون
Naseef, Hani
Moqadi, Ramzi
Qurt, Moammal
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-24
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Alogliptin benzoate, a member of dipeptidyl peptidase-4 inhibitors, is a recent drug developed by Takeda Pharmaceutical Company for the treatment of Type 2 diabetes; it potentiates the effect of incretin hormones through the inhibition of their degradation.
Alogliptin can be used alone or in combination therapy.
A new sensitive and rapid HPLC method was developed for the determination of alogliptin benzoate in bulk and pharmaceutical dosage forms; it was validated according to ICH and FDA guidelines.
The HPLC analysis was performed on the Agilent 1200 system equipped with a Hypersil Gold Thermo Scientific C18 (250 cm × 4.6 mm) 5 µm column, with a mixture of acetonitrile and ammonium carbonate buffer in the ratio of 55 : 45 v/v as the mobile phase, at the flow rate of 1.0 mL/min.
The detection was performed at the wavelength (λ) of 277, and the retention time of alogliptin benzoate was around 4 min.
The total run time was 6.0 min.
The calibration plot gave linear relationship over the concentration range of 85–306 µg/ml.
The LOD and LOQ were 0.03 and 0.09 μg, respectively.
The accuracy of the proposed method was determined by recovery studies and was found to be 100.3%.
The repeatability testing for both standard and sample solutions showed that the method is precise within the acceptable limits.
RSD% of the determination of precision was <2%.
The results of robustness and solutions stability studies were within the acceptable limits as well.
The proposed method showed excellent linearity, accuracy, precision, specificity, robustness, LOD, LOQ, and system suitability results within the acceptance criteria.
In addition, the main features of the developed method are low run time and retention time around 4 min.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Naseef, Hani& Moqadi, Ramzi& Qurt, Moammal. 2018. Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoate in Bulk and Tablets. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176198
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Naseef, Hani…[et al.]. Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoate in Bulk and Tablets. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176198
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Naseef, Hani& Moqadi, Ramzi& Qurt, Moammal. Development and Validation of an HPLC Method for Determination of Antidiabetic Drug Alogliptin Benzoate in Bulk and Tablets. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176198
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176198
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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