The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4+ T Cells
المؤلفون المشاركون
Käfer, Rudolf
Schmidtke, Lisa
Schrick, Katharina
Montermann, Evelyn
Bros, Matthias
Pautz, Andrea
Kleinert, Hartmut
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-14
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
The KH-type splicing regulatory protein (KSRP) is a RNA-binding protein, which regulates the stability of many mRNAs encoding immune-relevant proteins.
As KSRP regulates innate immune responses, for instance by the modulation of type I interferon mRNA stability, we were interested whether knockdown of the protein (KSRP-/-) interferes with T cell activation and polarization.
Polyclonally stimulated KSRP-/- CD4+ T cells proliferated at a higher extent and higher frequency and expressed the activation marker CD25 more than wild-type T cells.
In supernatants of stimulated KSRP-/- CD4+ T cells, levels of IL-5, IL-9, IL-10, and IL-13 were observed to be increased compared to those of the control group.
KSRP-/- CD8+ T cells showed no altered proliferative capacity upon polyclonal stimulation, but supernatants contained lower levels of interferon-γ.
Similar changes in the cytokine expression patterns were also detected in T cells derived from KSRP-/- mice undergoing arthritis induction indicative of a pathophysiological role of KSRP-dependent T cell polarization.
We demonstrated the direct binding of KSRP to the 3′ untranslated region of IL-13, IL-10, and IFN-γ mRNA in in vitro experiments.
Moreover, since IL-4 mRNA decay was reduced in KSRP-/- CD4+ T cells, we identify KSRP as a negative regulator of IL-4 expression.
These data indicate that overexpression of IL-4, which constitutes the primary inducer of Th2 polarization, may cause the Th2 bias of polyclonally stimulated KSRP-/- CD4+ T cells.
This is the first report demonstrating that KSRP is involved in the regulation of T cell responses.
We present strong evidence that T cells derived from KSRP-/- mice favor Th2-driven immune responses.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Käfer, Rudolf& Schmidtke, Lisa& Schrick, Katharina& Montermann, Evelyn& Bros, Matthias& Kleinert, Hartmut…[et al.]. 2019. The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4+ T Cells. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1176256
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Käfer, Rudolf…[et al.]. The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4+ T Cells. Journal of Immunology Research No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1176256
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Käfer, Rudolf& Schmidtke, Lisa& Schrick, Katharina& Montermann, Evelyn& Bros, Matthias& Kleinert, Hartmut…[et al.]. The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4+ T Cells. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1176256
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176256
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر