Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool
المؤلفون المشاركون
Wang, Bing
Gu, Jincui
Chen, Boyi
Xu, Chengfeng
Zheng, Hailing
Peng, Zhiqin
Zhou, Yang
Hu, Zhiwen
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-05
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The identification of ancient wool is of great importance in archaeology.
Despite lots of meaningful information can be achieved by conventional detection methods, that is, light and electron microscopy, spectroscopy, and chromatography, the efficacy is likely to be limited in the detection of ancient samples with contamination or severe degradation.
In this work, an immunoassay was proposed and performed for the identification of ancient wool.
First, a specific antibody, which has the benefits of low cost, easy operation, and extensive applicability, was developed directly through immunizing rabbits with complete antigen (keratin).
Then, an enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-labelled immunochromatographic strip (ICS) were developed to qualitatively identify the corresponding protein in ancient wool samples unearthed from Kazakhstan and China.
The anti-keratin antibody exhibited high sensitivity and specificity for the identification of modern and ancient wool.
The limit of detection (LOD) of the ELISA method was 10 ng/mL, and no cross-reactions with other interfering antigens have been noted.
It is concluded that the immunoassays are reliable methods for the identification of ancient wool.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Bing& Gu, Jincui& Chen, Boyi& Xu, Chengfeng& Zheng, Hailing& Peng, Zhiqin…[et al.]. 2018. Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176260
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Bing…[et al.]. Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1176260
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Bing& Gu, Jincui& Chen, Boyi& Xu, Chengfeng& Zheng, Hailing& Peng, Zhiqin…[et al.]. Development of an Enzyme-Linked Immunosorbent Assay and Gold-Labelled Immunochromatographic Strip Assay for the Detection of Ancient Wool. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176260
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176260
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر