UPLC-MSMS Method for Simultaneous Determination of Three Major Metabolites of Mequindox in Holothurian
المؤلفون المشاركون
Xu, Lihua
Li, Yanshen
Liu, Huihui
Ren, Chuanbo
Han, Dianfeng
Huang, Hui
Zou, Rongjie
Zhang, Huawei
Xu, Yingjiang
Gong, Xianghong
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-01
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
This study developed an ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the detection of three major metabolites of mequindox, including 3-methyl-quinoxaline-2-carboxylic acid, 1-desoxymequindox, and 1,4-bisdesoxymequindox (MQCA, 1-DMEQ, and BDMEQ), in holothurian.
Target analytes were simplified with ultrasound-assisted acidolysis extracted without complicated enzymolysis steps.
After that, each sample was centrifuged and purified by an Oasis MAX cartridge.
Then, the processed samples were separated and monitored by UPLC-MS/MS.
This developed method has been validated according to FDA criteria.
At fortified levels of 2, 10, and 20 μg/kg, recoveries ranged from 82.5% to 93.5% with the intraday RSD less than 7.27% and interday RSD less than 11.8%.
The limit of detection (LOD) of all the three metabolites ranged from 0.21 to 0.48 μg/kg, while the limit of quantification (LOQ) ranged from 0.79 to 1.59 μg/kg.
On application to commercial samples, 14 of 20 samples were detected positive for the three target analytes, with positive rate at 70 percentage.
The result indicated that this method was specific, sensitive, and suitable for the quantification and conformation of the three major metabolites of MEQ in holothurian.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Huihui& Ren, Chuanbo& Han, Dianfeng& Huang, Hui& Zou, Rongjie& Zhang, Huawei…[et al.]. 2018. UPLC-MSMS Method for Simultaneous Determination of Three Major Metabolites of Mequindox in Holothurian. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176272
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Huihui…[et al.]. UPLC-MSMS Method for Simultaneous Determination of Three Major Metabolites of Mequindox in Holothurian. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176272
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Huihui& Ren, Chuanbo& Han, Dianfeng& Huang, Hui& Zou, Rongjie& Zhang, Huawei…[et al.]. UPLC-MSMS Method for Simultaneous Determination of Three Major Metabolites of Mequindox in Holothurian. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176272
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176272
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر