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Development and Validation of an LC-MSMS Method and Comparison with a GC-MS Method to Measure Phenytoin in Human Brain Dialysate, Blood, and Saliva
المؤلفون المشاركون
Hösli, Raphael
König, Stefan
Mühlebach, Stefan F.
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-01
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Phenytoin (PHT) is one of the most often used critical dose drugs, where insufficient or excessive dosing can have severe consequences such as seizures or toxicity.
Thus, the monitoring and precise measuring of PHT concentrations in patients is crucial.
This study develops and validates an LC-MS/MS method for the measurement of phenytoin concentrations in different body compartments (i.e., human brain dialysate, blood, and saliva) and compares it with a formerly developed GC-MS method that measures PHT in the same biological matrices.
The two methods are evaluated and compared based on their analytical performance, appropriateness to analyze human biological samples, including corresponding extraction and cleanup procedures, and their validation according to ISO 17025/FDA Guidance for Industry.
The LC-MS/MS method showed a higher performance compared with the GC-MS method.
The LC-MS/MS was more sensitive, needed a smaller sample volume (25 µL) and less chemicals, was less time consuming (cleaning up, sample preparation, and analysis), and resulted in a better LOD (<1 ng/mL)/LOQ (10 ng/mL).
The calibration curve of the LC-MS/MS method (10–2000 ng/mL) showed linearity over a larger range with correlation coefficients r2 > 0.995 for all tested matrices (blood, saliva, and dialysate).
For larger sample numbers as in pharmacokinetic/pharmacodynamic studies and for bedside as well as routine analyses, the LC-MS/MS method offers significant advantages over the GC-MS method.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hösli, Raphael& König, Stefan& Mühlebach, Stefan F.. 2018. Development and Validation of an LC-MSMS Method and Comparison with a GC-MS Method to Measure Phenytoin in Human Brain Dialysate, Blood, and Saliva. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1176565
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hösli, Raphael…[et al.]. Development and Validation of an LC-MSMS Method and Comparison with a GC-MS Method to Measure Phenytoin in Human Brain Dialysate, Blood, and Saliva. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1176565
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hösli, Raphael& König, Stefan& Mühlebach, Stefan F.. Development and Validation of an LC-MSMS Method and Comparison with a GC-MS Method to Measure Phenytoin in Human Brain Dialysate, Blood, and Saliva. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1176565
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176565
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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