Sm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host
المؤلفون المشاركون
Ribeiro, R. I. M. A.
Bernardes, Wilma Patrícia de Oliveira Santos
de Araújo, Juliano Michel
Carvalho, Gardênia Braz
Alves, Clarice Carvalho
de Moura Coelho, Aline Thaynara
Dutra, Isabela Thamara Sabino
Teixeira, Sueleny Silva Ferreira
de Moraes Mourão, Marina
da Silva-Pereira, Rosiane Aparecida
Fonseca, Cristina Toscano
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-01
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Sm16 is an immunomodulatory protein that seems to play a key role in the suppression of the cutaneous inflammatory response during Schistosoma mansoni penetration of the skin of definitive hosts.
Therefore, Sm16 represents a potential target for protective immune responses induced by vaccination.
In this work, we generated the recombinant protein rSm16 and produced polyclonal antibodies against this protein to evaluate its expression during different parasite life-cycle stages and its location on the surface of the parasite.
In addition, we analyzed the immune responses elicited by immunization with rSm16 using two different vaccine formulations, as well as its ability to induce protection in Balb/c mice.
In order to explore the biological function of Sm16 during the course of experimental infection, RNA interference was also employed.
Our results demonstrated that Sm16 is expressed in cercaria and schistosomula and is located in the schistosomula surface.
Despite humoral and cellular immune responses triggered by vaccination using rSm16 associated with either Freund’s or alum adjuvants, immunized mice presented no reduction in either parasite burden or parasite egg laying.
Knockdown of Sm16 gene expression in schistosomula resulted in decreased parasite size in vitro but had no effect on parasite survival or egg production in vivo.
Thus, our findings demonstrate that although the vaccine formulations used in this study succeeded in activating immune responses, these failed to promote parasite elimination.
Finally, we have shown that Sm16 is not vital for parasite survival in the definitive host and hence may not represent a suitable target for vaccine development.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bernardes, Wilma Patrícia de Oliveira Santos& de Araújo, Juliano Michel& Carvalho, Gardênia Braz& Alves, Clarice Carvalho& de Moura Coelho, Aline Thaynara& Dutra, Isabela Thamara Sabino…[et al.]. 2019. Sm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1176582
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bernardes, Wilma Patrícia de Oliveira Santos…[et al.]. Sm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host. Journal of Immunology Research No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1176582
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bernardes, Wilma Patrícia de Oliveira Santos& de Araújo, Juliano Michel& Carvalho, Gardênia Braz& Alves, Clarice Carvalho& de Moura Coelho, Aline Thaynara& Dutra, Isabela Thamara Sabino…[et al.]. Sm16, A Schistosoma mansoni Immunomodulatory Protein, Fails to Elicit a Protective Immune Response and Does Not Have an Essential Role in Parasite Survival in the Definitive Host. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1176582
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1176582
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر