![](/images/graphics-bg.png)
Intranasal Application of Budesonide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Suppressing Nucleotide-Binding Oligomerization Domain-Like Receptor Family, Pyrin Domain-Containing 3 Inflammasome Activation in Mice
المؤلفون المشاركون
Dong, Liang
Zhu, Yu-Hang
Liu, De-Xing
Li, Juan
Zhao, Peng-Cheng
Zhong, Yuan-Ping
Chen, Yong-Qin
Xu, Wei
Zhu, Zhao-Qiong
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-02-27
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Aim.
To investigate the protective effects of budesonide against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in a murine model and its underlying mechanism.
Methods.
Adult male C57BL/6 mice were divided into three groups: control, ALI, and ALI + budesonide groups.
LPS (5 mg/kg) was intratracheally injected to induce ALI in mice.
Budesonide (0.5 mg/kg) was intranasally given 1 h before LPS administration in the ALI + budesonide group.
Twelve hours after LPS administration, all mice were sacrificed.
Hematoxylin-eosin staining and pathological scores were used to evaluate pathological injury.
Bronchoalveolar lavage was performed.
The numbers of total cells, neutrophils, and macrophages in the bronchoalveolar lavage fluid (BALF) were counted.
Enzyme-linked immunosorbent assay was employed to detect the proinflammatory cytokines in BALF and serum, including tumor necrosis factor- (TNF-) α, monocyte chemoattractant protein- (MCP-) 1, and interleukin- (IL-) 1β.
The expression of the nucleotide-binding oligomerization domain-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome was detected by western blotting.
A lethal dose of LPS (40 mg/kg, intraperitoneally) was injected to evaluate the effects of budesonide on survival rates.
Results.
Budesonide pretreatment dramatically attenuated pathological injury and reduced pathological scores in mice with ALI.
Budesonide pretreatment obviously reduced the numbers of total cells, neutrophils, and macrophages in the BALF of mice with ALI.
Additionally, budesonide dramatically reduced TNF-α and MCP-1 expression in the BALF and serum of mice with ALI.
Budesonide significantly suppressed NLRP3 and pro-caspase-1 expression in the lung and reduced IL-1β content in the BALF, indicating that budesonide inhibited the activation of the NLRP3 inflammasome.
Furthermore, we found that budesonide improved the survival rates of mice with ALI receiving a lethal dose of LPS.
Conclusion.
Suppression of NLRP3 inflammasome activation in mice via budesonide attenuated lung injury induced by LPS in mice with ALI.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dong, Liang& Zhu, Yu-Hang& Liu, De-Xing& Li, Juan& Zhao, Peng-Cheng& Zhong, Yuan-Ping…[et al.]. 2019. Intranasal Application of Budesonide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Suppressing Nucleotide-Binding Oligomerization Domain-Like Receptor Family, Pyrin Domain-Containing 3 Inflammasome Activation in Mice. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1180739
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dong, Liang…[et al.]. Intranasal Application of Budesonide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Suppressing Nucleotide-Binding Oligomerization Domain-Like Receptor Family, Pyrin Domain-Containing 3 Inflammasome Activation in Mice. Journal of Immunology Research No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1180739
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dong, Liang& Zhu, Yu-Hang& Liu, De-Xing& Li, Juan& Zhao, Peng-Cheng& Zhong, Yuan-Ping…[et al.]. Intranasal Application of Budesonide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Suppressing Nucleotide-Binding Oligomerization Domain-Like Receptor Family, Pyrin Domain-Containing 3 Inflammasome Activation in Mice. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1180739
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1180739
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)