Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes
المؤلفون المشاركون
Tian, Hong
Yao, Wenbing
Gao, Xiangdong
Jiang, Liangliang
Jiang, Tao
Luo, Jianhua
Kang, Yanliang
Tong, Yue
Song, Xiaoda
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-30
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Single B cell antibody technology is a method for isolating antigen-specific B cells from human peripheral blood and obtaining antibody genes in developing antibody drugs.
However, owing to immune tolerance to autoantigen, human autoantigen-specific B cells are difficult to acquire by conventional single B cell technology.
In this study, we constructed a nitrated T-cell epitope named NitraTh by incorporating p-nitrophenylalanine into a universal T helper epitope.
NitraTh had enhanced ability to activate CD4+ T cells and can be recognized by CD4+ T cells with different HLA class II haplotypes.
This NitraTh can also break immune tolerance to autoantigens, such as human epidermal growth factor receptor 2 (HER2) and cannabinoid receptor 1, and induce strong specific IgM+ B cell responses in vitro.
HER2-NitraTh vaccine can also stimulate the generation of HER2-specific IgG+ B cells in human immune system mice, which was established by cotransplanting lymphocytes and autologous dendritic cells in immunodeficient mice.
We obtained 30 fully human IgG antibody genes by sorting single B cells from the human immune system mice immunized with HER2-NitraTh vaccine.
The analysis of antibody genes showed that sorted B cells underwent the extensive somatic mutation of the antibody genes.
We randomly selected eight genes for cloning, six of which expressed antibodies that can bind to HER2.
Hence, we provided a convenient and effective method in acquiring fully human antibody genes against self-proteins, which can be used in developing therapeutic antibody drugs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiang, Liangliang& Jiang, Tao& Luo, Jianhua& Kang, Yanliang& Tong, Yue& Song, Xiaoda…[et al.]. 2019. Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1180865
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiang, Liangliang…[et al.]. Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes. Journal of Immunology Research No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1180865
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiang, Liangliang& Jiang, Tao& Luo, Jianhua& Kang, Yanliang& Tong, Yue& Song, Xiaoda…[et al.]. Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1180865
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1180865
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر