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Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement
المؤلفون المشاركون
Kishikawa, Akiko
Ogawa, Saika
Qi, Jiawei
Shen, Wei-Ren
Ohori, Fumitoshi
Noguchi, Takahiro
Marahleh, Aseel
Nara, Yasuhiko
Mizoguchi, Itaru
Kitaura, Hideki
المصدر
Journal of Immunology Research
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-24
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Osteocytes are abundant cells in bone, which contribute to bone maintenance.
Osteocytes express receptor activator of nuclear factor kappa-B ligand (RANKL) and regulate osteoclast formation.
Orthodontic tooth movement (OTM) occurs by osteoclast resorption of alveolar bone.
Osteocyte-derived RANKL is critical in bone resorption during OTM.
Additionally, tumor necrosis factor-α (TNF-α) is important in osteoclastogenesis during OTM.
Sclerostin has been reported to enhance RANKL expression in the MLO-Y4 osteocyte-like cell line.
This study investigated the effect of TNF-α on sclerostin expression in osteocytes during OTM.
In vitro analysis of primary osteocytes, which were isolated from DMP1-Topaz mice by sorting the Topaz variant of GFP-positive cells, revealed that SOST mRNA expression was increased when osteocytes were cultured with TNF-α and that RANKL mRNA expression was increased when osteocytes were cultured with sclerostin.
Moreover, the number of TRAP-positive cells was increased in osteocytes and osteoclast precursors cocultured with sclerostin.
In vivo analysis of mouse calvariae that had been subcutaneously injected with phosphate-buffered saline (PBS) or TNF-α revealed that the number of TRAP-positive cells and the percentage of sclerostin-positive osteocytes were higher in the TNF-α group than in the PBS group.
Furthermore, the level of SOST mRNA was increased by TNF-α.
As an OTM model, a Ni-Ti closed-coil spring connecting the upper incisors and upper-left first molar was placed to move the first molar to the mesial direction in wild-type (WT) mice and TNF receptor 1- and 2-deficient (TNFRsKO) mice.
After 6 days of OTM, the percentage of sclerostin-positive osteocytes on the compression side of the first molar in TNFRsKO mice was lower than that in WT mice.
In this study, TNF-α increased sclerostin expression in osteocytes, and sclerostin enhanced RANKL expression in osteocytes.
Thus, TNF-α may play an important role in sclerostin expression in osteocytes and enhance osteoclast formation during OTM.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ohori, Fumitoshi& Kitaura, Hideki& Marahleh, Aseel& Kishikawa, Akiko& Ogawa, Saika& Qi, Jiawei…[et al.]. 2019. Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1181159
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ohori, Fumitoshi…[et al.]. Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement. Journal of Immunology Research No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1181159
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ohori, Fumitoshi& Kitaura, Hideki& Marahleh, Aseel& Kishikawa, Akiko& Ogawa, Saika& Qi, Jiawei…[et al.]. Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1181159
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1181159
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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