Plane Animation Simulation of the Interaction between Carbon Nanomaterials and Cell Lysosomes

الملخص EN

With the continuous development of cell dynamics, Raman scattering has become more and more common in the application of cell imaging and dynamic changes in chemical substances.

This research mainly discusses the plane animation simulation process of the interaction between carbon nanomaterials and cell lysosomes.

Twenty parallel HeLa cells were seeded on 40 mm imaging scaffolds.

The 15% bovine placental serum cell culture cells are placed in a thermostat for 12 hours at a CO2 concentration of 6%.

After washing 4 times, 20 μL of the dual control system is added to the confocal dish, and cycle optimization culture is performed at 2, 4, 6, and 8 hours.

It is incubated for 10 hours, 20 hours, and 30 hours (35°C, 6% CO2).

Next, the HeLa cells were taken out and seeded on three 30 mm confocal cell culture dishes.

CCl4 is added to the initial confocal Petri dish.

After heating for 30 minutes, the nanoparticle system is added to the two confocal Petri dishes and circulated within an appropriate time.

After washing with PBS, the SERS signal in the cells was imaged with a laser confocal Raman microscope, and the excitation channels were GFP 475 and Cy3 channels.

LysoTrackerRed (2p μM) was used for local experiments of cell isotope.

Deoxygen (2p μM) was used to induce cell death, and the pH changes in lysosomes in cells were imaged in real time with a confocal microscope.

Two distinct peaks in the Raman spectrum were observed at 1246 cm−1 and 1543 cm−1.

The research results show that the carbon nanomaterial synthesized by a simple method at room temperature has high stability and is suitable for analysis and detection of imaging with cells as the target.