RNA-Seq Based Transcriptome Analysis of the Type I Interferon Host Response upon Vaccinia Virus Infection of Mouse Cells
المؤلفون المشاركون
Hernáez, Bruno
Alonso, Graciela
Alonso-Lobo, Juan Manuel
Rastrojo, Alberto
Fischer, Cornelius
Sauer, Sascha
Aguado, Begoña
Alcamí, Antonio
المصدر
Journal of Immunology Research
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-02-09
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Vaccinia virus (VACV) encodes the soluble type I interferon (IFN) binding protein B18 that is secreted from infected cells and also attaches to the cell surface, as an immunomodulatory strategy to inhibit the host IFN response.
By using next generation sequencing technologies, we performed a detailed RNA-seq study to dissect at the transcriptional level the modulation of the IFN based host response by VACV and B18.
Transcriptome profiling of L929 cells after incubation with purified recombinant B18 protein showed that attachment of B18 to the cell surface does not trigger cell signalling leading to transcriptional activation.
Consistent with its ability to bind type I IFN, B18 completely inhibited the IFN-mediated modulation of host gene expression.
Addition of UV-inactivated virus particles to cell cultures altered the expression of a set of 53 cellular genes, including genes involved in innate immunity.
Differential gene expression analyses of cells infected with replication competent VACV identified the activation of a broad range of host genes involved in multiple cellular pathways.
Interestingly, we did not detect an IFN-mediated response among the transcriptional changes induced by VACV, even after the addition of IFN to cells infected with a mutant VACV lacking B18.
This is consistent with additional viral mechanisms acting at different levels to block IFN responses during VACV infection.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hernáez, Bruno& Alonso, Graciela& Alonso-Lobo, Juan Manuel& Rastrojo, Alberto& Fischer, Cornelius& Sauer, Sascha…[et al.]. 2017. RNA-Seq Based Transcriptome Analysis of the Type I Interferon Host Response upon Vaccinia Virus Infection of Mouse Cells. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1181904
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hernáez, Bruno…[et al.]. RNA-Seq Based Transcriptome Analysis of the Type I Interferon Host Response upon Vaccinia Virus Infection of Mouse Cells. Journal of Immunology Research No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1181904
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hernáez, Bruno& Alonso, Graciela& Alonso-Lobo, Juan Manuel& Rastrojo, Alberto& Fischer, Cornelius& Sauer, Sascha…[et al.]. RNA-Seq Based Transcriptome Analysis of the Type I Interferon Host Response upon Vaccinia Virus Infection of Mouse Cells. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1181904
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1181904
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر