Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response
المؤلفون المشاركون
Wan, Chunping
Xu, Shikui
Zuo, Aixue
Guo, Zengjun
المصدر
Journal of Immunology Research
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-15
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The present study was designed to assess the antiarthritic potential of ECF in collagen-induced arthritis (CIA) and explore its underlying mechanism.
Methods.
In vitro, lymphocyte proliferation assay was measured by CCK-8 kit.
In vivo, the therapeutic potential of ECF on CIA was investigated; surface marker, Treg cell, and intracellular cytokines (IL-17A and IFN-γ) were detected by flow cytometry.
Th1 cell differentiation assay was performed, and mRNA expression in interferon-γ-related signaling was examined by q-PCR analysis.
Results.
In vitro, ECF markedly inhibited the proliferation of splenocytes in response to ConA and anti-CD3.
In vivo, ECF treatment reduced the severity of CIA, inhibited IFN-γ and IL-6 secretion, and decreased the proportion of CD11b+Gr-1+ splenic neutrophil.
Meanwhile, ECF treatment significantly inhibited the IFN-γ expression in CD4+T cell without obviously influencing the development of Th17 cells and T regulatory cells.
In vitro, ECF suppressed the differentiation of naive CD4+ T cells into Th1.
Furthermore, ECF intensely blocked the transcriptional expression in interferon-γ-related signaling, including IFN-γ, T-bet, STAT1, and STAT4.
Conclusion.
Our results indicated that ECF exerted antiarthritic potential in collagen-induced arthritis by suppressing Th1 immune response and interferon-γ-related signaling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Shikui& Zuo, Aixue& Guo, Zengjun& Wan, Chunping. 2017. Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182072
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Shikui…[et al.]. Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response. Journal of Immunology Research No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1182072
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Shikui& Zuo, Aixue& Guo, Zengjun& Wan, Chunping. Ethyl Caffeate Ameliorates Collagen-Induced Arthritis by Suppressing Th1 Immune Response. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182072
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1182072
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر