Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects
المؤلفون المشاركون
Hussain, Syed Zajif
Shahzad, Asma
Saeed, Hamid
Iqtedar, Mehwish
Kaleem, Afshan
Abdullah, Roheena
Sharif, Saima
Naz, Shagufta
Saleem, Faiza
Aihetasham, Ayesha
Chaudhary, Asma
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-02-24
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
The biogenesis of silver nanoparticles by fungi is an ecologically clean and nontoxic method compared to other physical and chemical methods.
Thus, we aimed to discuss the mycosynthesis of extracellular size-controlled AgNPs.
After comprehensive screening, Aspergillus fumigatus BTCB10 (KY486782) was selected for the synthesis of AgNPs of controlled size.
Characterization was performed by UV-Vis spectrophotometer, Zetasizer, X-Ray Diffraction (XRD), FTIR (Fourier-transform infrared), Atomic Force Microscopy (AFM), and Scanning Electron Microscopy (SEM) along with functional assays—antibacterial and MTT assays.
Data suggested that under optimized conditions, i.e., temperature 25°C, AgNO3 concentration 1 mM, biomass 7 g, fungal culture age 7 days, pH 6, ratio of cell-free filtrate (CFF)/silver nitrate (3 : 2), NaCl 20%, and under dark light, the smallest size AgNPs of 0.681 nm with 100% monodispersity was obtained as evident by a zeta potential of -23.4 mV, UV-Vis band at 400 nm, and the presence of O-H and C=O groups confirmed by ATR-FTIR; XRD revealed the crystalline nature of AgNPs; additionally, cube-shaped AgNPs were revealed by Scanning Electron Microscopy (SEM).
Moreover, synthesized AgNPs exhibited antibacterial activity against multidrug-resistant bacterial strains, notably, Klebsiella pneumoniae BTCB04, Acinetobacter BTCB05, Pseudomonas aeruginosa BTCB01, and Escherichia coli BTCB03, while maximum 7-fold was observed with Acinetobacter BTCB05.
AgNPs demonstrated no cytotoxic activity against HepG2 cells; however, in combination with cisplatin, antiproliferative and cytotoxic effects became more evident and significant in comparison to control and as single agent.
Taken together, the data suggested that economical and smallest size AgNPs can be biosynthesized from Aspergillus fumigatus BTCB10 and be used as antibacterial and antiproliferative agents in combination with current drugs against clinically relevant multiple drug-resistant bacterial and tumoral cells.
Further studies are required to confirm their effects employing in vivo disease models.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shahzad, Asma& Saeed, Hamid& Iqtedar, Mehwish& Hussain, Syed Zajif& Kaleem, Afshan& Abdullah, Roheena…[et al.]. 2019. Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects. Journal of Nanomaterials،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1182427
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shahzad, Asma…[et al.]. Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects. Journal of Nanomaterials No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1182427
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shahzad, Asma& Saeed, Hamid& Iqtedar, Mehwish& Hussain, Syed Zajif& Kaleem, Afshan& Abdullah, Roheena…[et al.]. Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects. Journal of Nanomaterials. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1182427
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1182427
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر