![](/images/graphics-bg.png)
Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage
المؤلفون المشاركون
Tsai, Yi-Chih
Wang, Yi-Hsiang
Liu, Yin-Chang
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-01
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
UVC irradiation-caused DNA lesions are repaired in mammalian cells solely by nucleotide excision repair (NER), which consists of sequential events including initial damage recognition, dual incision of damage site, gap-filling, and ligation.
We have previously shown that gap-filling during the repair of UV-induced DNA lesions may be delayed by a subsequent treatment of oxidants or prooxidants such as hydrogen peroxide, flavonoids, and colcemid.
We considered the delay as a result of competition for limiting protein/enzyme factor(s) during repair synthesis between NER and base excision repair (BER) induced by the oxidative chemicals.
In this report, using colcemid as oxidative stress inducer, we showed that colcemid-caused delay of gap-filling during the repair of UV-induced DNA lesions was attenuated by overexpression of PCNA but not ligase-I.
PCNA knockdown, as expected, delayed the gap-filling of NER but also impaired the repair of oxidative DNA damage.
Fen-1 knockdown, however, did not affect the repair of oxidative DNA damage, suggesting repair of oxidative DNA damage is not of long patch BER.
Furthermore, overexpression of XRCC1 delayed the gap-filling, and presumably increase of XRCC1 pulls PCNA away from gap-filling of NER for BER, consistent with our hypothesis that delay of gap-filling of NER attributes the competition between NER and BER.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tsai, Yi-Chih& Wang, Yi-Hsiang& Liu, Yin-Chang. 2017. Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage. Journal of Nucleic Acids،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1182479
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tsai, Yi-Chih…[et al.]. Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage. Journal of Nucleic Acids No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1182479
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tsai, Yi-Chih& Wang, Yi-Hsiang& Liu, Yin-Chang. Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage. Journal of Nucleic Acids. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1182479
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1182479
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)