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High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression
المؤلفون المشاركون
Qi, Jie
Wu, Qichao
Cheng, Qian
Chen, Xiangyuan
Zhu, Minmin
Miao, Changhong
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-24
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Cyclooxygenase 2 (COX2) and inducible nitric oxide synthase (iNOS) overexpression results in endothelial apoptosis, thus mediating vascular endothelial injury in hyperglycaemia.
E26 transformation-specific sequence transcription factor-1 (ESE-1), which belongs to the E26 transformation-specific family of transcription factors, has been demonstrated to be involved in COX2 and iNOS gene transcription.
Our previous study indicated that SET domain-containing protein 8 (SETD8) downregulation is involved in high glucose-mediated endothelial inflammation in human umbilical vein endothelial cells (HUVECs).
Here, we report that SETD8 plays a major role in hyperglycaemia-induced COX2 and iNOS expression.
In HUVECs, upregulation of ESE-1 expression was related to high glucose-mediated apoptosis and COX2 and iNOS expression.
High glucose inhibited SETD8 expression, and overexpression of SETD8 diminished the effects of high glucose treatment.
Consistently, RNA silencing of SETD8 led to the opposite effect.
Furthermore, SETD8 was found to interact with specificity protein 1 (SP1).
Blockade of SP1 protected against high glucose-mediated endothelial injury.
Mechanistically, we showed that H4K20me1, a downstream target of SETD8, and SP1 were enriched at the ESE-1 promoter region by ChIP assay.
Luciferase reporter assays indicated that SETD8 overexpression attenuated ESE-1 promoter activity and augmented the inhibitory effect of siSP1 on ESE-1 promoter activity.
In general, our data indicate that SETD8 interacts with SP1 to coregulate ESE-1 expression, which is involved in hyperglycaemia-mediated endothelial apoptosis in HUVECs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Qi, Jie& Wu, Qichao& Cheng, Qian& Chen, Xiangyuan& Zhu, Minmin& Miao, Changhong. 2020. High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1182938
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Qi, Jie…[et al.]. High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression. Journal of Diabetes Research No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1182938
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Qi, Jie& Wu, Qichao& Cheng, Qian& Chen, Xiangyuan& Zhu, Minmin& Miao, Changhong. High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1182938
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1182938
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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