Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency

المؤلفون المشاركون

Tang, Jun
Xu, Wei
Sun, Zilin
Zhou, Yunting
Zhou, Junming
Zhang, Yumin
Sun, Bo
Wang, Xiaohang
Chen, Yang

المصدر

Journal of Diabetes Research

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-01-22

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الأمراض
الطب البشري

الملخص EN

Aims.

The underlying mechanisms involved in Vitamin A- (VA-) related changes in glucose metabolic disorders remain unclear.

Recent evidence suggests that intestinal microbiota is closely linked to the metabolic syndrome.

Here, we explored whether and how intestinal microbiota affects glucose homeostasis in VA-deficient diet-fed mice.

Methods.

Six-week-old male C57BL/6 mice were randomly placed on either a VA-sufficient (VAS) or VA-deficient (VAD) diet for 10 weeks.

Subsequently, a subclass of the VAD diet-fed mice was switched to a VA-deficient rescued (VADR) diet for an additional 8 weeks.

The glucose metabolic phenotypes of the mice were assessed using glucose tolerance tests and immunohistochemistry staining.

Changes in intestinal microbiota were assessed using 16S gene sequencing.

The intestinal morphology, intestinal permeability, and inflammatory response activation signaling pathway were assessed using histological staining, western blots, quantitative-PCR, and enzyme-linked immunosorbent assays.

Results.

VAD diet-fed mice displayed reduction of tissue VA levels, increased area under the curve (AUC) of glucose challenge, reduced glucose-stimulated insulin secretion, and loss of β cell mass.

Redundancy analysis showed intestinal microbiota diversity was significantly associated with AUC of glucose challenge and β cell mass.

VAD diet-driven changes in intestinal microbiota followed the inflammatory response with increased intestinal permeability and higher mRNA expression of intestinal inflammatory cytokines through nuclear factor-κB signaling pathway activation.

Reintroduction of dietary VA to VAD diet-fed mice restored tissue VA levels, endocrine hormone profiles, and inflammatory response, which are similar to those observed following VAS-controlled changes in intestinal microbiota.

Conclusions.

We found intestinal microbiota effect islet function via controlling intestinal inflammatory phenotype in VAD diet-fed mice.

Intestinal microbiota influences could be considered as an additional mechanism for the effect of endocrine function in a VAD diet-driven mouse model.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zhou, Yunting& Zhou, Junming& Zhang, Yumin& Tang, Jun& Sun, Bo& Xu, Wei…[et al.]. 2020. Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency. Journal of Diabetes Research،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1182943

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zhou, Yunting…[et al.]. Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency. Journal of Diabetes Research No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1182943

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zhou, Yunting& Zhou, Junming& Zhang, Yumin& Tang, Jun& Sun, Bo& Xu, Wei…[et al.]. Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency. Journal of Diabetes Research. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1182943

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1182943