Identification of Potential Therapeutic Targets in the Liver of Pioglitazone-Treated Type 2 Diabetes Sprague-Dawley Rats via Expression Profile Chip and iTRAQ Assay
المؤلفون المشاركون
Li, Changyu
Lu, Zhong-Xia
Xu, Wen-Jun
Wu, Yang-Sheng
Chen, Yi-Tao
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-18
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The aim of the present study was to identify key antidiabetic nodes in the livers of pioglitazone-treated type 2 diabetes mellitus Sprague-Dawley rats by transcriptomic and proteomic analysis.
Rats were randomly divided into the control, the diabetes model, and the pioglitazone-treated groups.
After treatment with pioglitazone for 11 weeks, the effects on fasting blood glucose, body weight, and blood biochemistry parameters were evaluated.
Microarray and iTRAQ analysis were used to determine the differentially expressed genes/proteins in rat livers.
1.5-fold changes in gene expression and 1.2-fold changes in protein were set as the screening criteria.
After treatment with pioglitazone for 11 weeks, fasting blood glucose in pioglitazone-treated rats was significantly lower than that in the model group.
There was a tendency for pioglitazone to reduce TC, TG, TP, ALB, BUN, and HDL-c levels.
Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) were applied to analyze differentially expressed genes/proteins.
Furthermore, Western blotting and RT-qPCR were used to validate the results of microarray and iTRAQ.
In conclusion, Cyp7a1, Cp, and RT1-EC2 are differentially expressed genes/proteins since they showed a similar trend in rats in the model group and the pioglitazone-treated group.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Zhong-Xia& Xu, Wen-Jun& Wu, Yang-Sheng& Li, Changyu& Chen, Yi-Tao. 2018. Identification of Potential Therapeutic Targets in the Liver of Pioglitazone-Treated Type 2 Diabetes Sprague-Dawley Rats via Expression Profile Chip and iTRAQ Assay. Journal of Diabetes Research،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1183931
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Zhong-Xia…[et al.]. Identification of Potential Therapeutic Targets in the Liver of Pioglitazone-Treated Type 2 Diabetes Sprague-Dawley Rats via Expression Profile Chip and iTRAQ Assay. Journal of Diabetes Research No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1183931
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Zhong-Xia& Xu, Wen-Jun& Wu, Yang-Sheng& Li, Changyu& Chen, Yi-Tao. Identification of Potential Therapeutic Targets in the Liver of Pioglitazone-Treated Type 2 Diabetes Sprague-Dawley Rats via Expression Profile Chip and iTRAQ Assay. Journal of Diabetes Research. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1183931
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1183931
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر