DNA Hypermethylation Downregulates Telomerase Reverse Transcriptase (TERT) during H. pylori-Induced Chronic Inflammation
المؤلفون المشاركون
Bussière, Françoise I.
Michel, Valérie
Fernandes, Julien
Costa, Lionel
Camilo, Vania
Nigro, Giulia
De Reuse, Hilde
Fiette, Laurence
Touati, Eliette
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-12-31
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Helicobacter pylori infection causes chronic gastritis and is the major risk factor of gastric cancer.
H.
pylori induces a chronic inflammation-producing reactive oxygen species (ROS) which is a source of chromosome instabilities and contributes to the development of malignancy.
H.
pylori also promotes DNA hypermethylation, known to dysregulate essential genes that maintain genetic stability.
The maintenance of telomere length by telomerase is essential for chromosome integrity.
Telomerase reverse transcriptase (TERT) is the catalytic component of telomerase activity and an important target during host-pathogen interaction.
We aimed to investigate the consequences of H.
pylori on the regulation of TERT gene expression and telomerase activity.
In vitro, hTERT mRNA levels and telomerase activity were analysed in H.
pylori-infected human gastric epithelial cells.
In addition, C57BL/6 and INS-GAS mice were used to investigate the influence of H.
pylori-induced inflammation on TERT levels.
Our data demonstrated that, in vitro, H.
pylori inhibits TERT gene expression and decreases the telomerase activity.
The exposure of cells to lycopene, an antioxidant compound, restores TERT levels in infected cells, indicating that ROS are implicated in this downregulation.
In vivo, fewer TERT-positive cells are observed in gastric tissues of infected mice compared to uninfected, more predominantly in the vicinity of large aggregates of lymphocytes, suggesting an inflammation-mediated regulation.
Furthermore, H.
pylori appears to downregulate TERT gene expression through DNA hypermethylation as shown by the restoration of TERT transcript levels in cells treated with 5′-azacytidine, an inhibitor of DNA methylation.
This was confirmed in infected mice, by PCR-methylation assay of the TERT gene promoter.
Our data unraveled a novel way for H.
pylori to promote genome instabilities through the inhibition of TERT levels and telomerase activity.
This mechanism could play an important role in the early steps of gastric carcinogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bussière, Françoise I.& Michel, Valérie& Fernandes, Julien& Costa, Lionel& Camilo, Vania& Nigro, Giulia…[et al.]. 2019. DNA Hypermethylation Downregulates Telomerase Reverse Transcriptase (TERT) during H. pylori-Induced Chronic Inflammation. Journal of Oncology،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1184302
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bussière, Françoise I.…[et al.]. DNA Hypermethylation Downregulates Telomerase Reverse Transcriptase (TERT) during H. pylori-Induced Chronic Inflammation. Journal of Oncology No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1184302
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bussière, Françoise I.& Michel, Valérie& Fernandes, Julien& Costa, Lionel& Camilo, Vania& Nigro, Giulia…[et al.]. DNA Hypermethylation Downregulates Telomerase Reverse Transcriptase (TERT) during H. pylori-Induced Chronic Inflammation. Journal of Oncology. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1184302
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1184302
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر