Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells
المؤلفون المشاركون
Nediani, Chiara
Peppicelli, Silvia
Ruzzolini, Jessica
Bianchini, Francesca
Andreucci, Elena
Laurenzana, Anna
Margheri, Francesca
Fibbi, Gabriella
Calorini, Lido
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-02
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Melanoma is characterized by a low extracellular pH, which contributes to the development of an aggressive phenotype characterized by several properties as the switch to an epithelial-to-mesenchymal program, the increase of apoptotic resistance, and the migratory ability together with the development of drug resistance.
Here, we demonstrate that melanoma cells grown in low pH medium (pH 6.7) for a short (24 hours) or long (at least 3 months) period equally express an anoikis resistance profile.
Anoikis is a form of apoptosis prompted by loss of adhesion, particularly requested by aggressive cancer cells to metastasize.
Anoikis resistance was ascertained in acidic melanoma cells either grown in agarose-coated plates or incubated in rocking conditions.
Both analyses indicate that acidic cells were more able to survive in a nonadherent condition than cells grown in standard pH, an effect resulting in a more cloning efficiency and migratory ability.
Ability to survive during rocking was inhibited using mTOR/NF-kB inhibitors.
Finally, we checked whether characteristics related to the in vitro anoikis resistance acquired by acidic melanoma cells might be also suitable for in vivo challenge.
We injected acidic melanoma cells into blood stream, and then we verify how many cells survived in blood after 15 min from the injection.
Only acidic cells, transient and chronic, survived, whereas melanoma cells grown in standard pH medium did not.
Overall, we have had the opportunity to demonstrate that low extracellular pH represents an additional mechanism able to promote an anoikis resistance in solid tumors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Peppicelli, Silvia& Ruzzolini, Jessica& Bianchini, Francesca& Andreucci, Elena& Nediani, Chiara& Laurenzana, Anna…[et al.]. 2019. Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells. Journal of Oncology،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1184564
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Peppicelli, Silvia…[et al.]. Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells. Journal of Oncology No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1184564
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Peppicelli, Silvia& Ruzzolini, Jessica& Bianchini, Francesca& Andreucci, Elena& Nediani, Chiara& Laurenzana, Anna…[et al.]. Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells. Journal of Oncology. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1184564
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1184564
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر