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Establishment and Application of a Method for the Determination of Ganoderic Acid A
المؤلفون المشاركون
Li, Shengyun
Yuan, Yaowu
Yu, Chenchen
Gao, Hao
Tan, Jianxin
Tian, Yiling
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-30
دولة النشر
مصر
عدد الصفحات
7
الملخص EN
A method for the quantitative determination of ganoderic acid A was constructed using the principle of indirect competitive enzyme-linked immunosorbent assay (ELISA), and this method was used to determine the ganoderic A contents of Ganoderma lucidum samples in the market.
The conjugate of ganoderic acid A and bovine serum albumin was used for four rounds of immunization on test rabbits to obtain rabbit antiganoderic acid A antibody IgG.
The enzyme-labeled plate was coated with the conjugate of ganoderic acid A and ovalbumin.
The first stage reaction in the indirect competitive ELISA was that the conjugate of ganoderic acid A in the sample competed with the conjugate coated on the enzyme-labeled plate to bind rabbit antibodies.
The second stage reaction was the combination of goat anti-rabbit IgG–horseradish peroxidase and rabbit antiganoderic acid A antibody IgG.
The results of the determination of ganoderic acid A standard by this method showed that the coefficient of variation of repeated wells in the group was <5%, the detection limit of ganoderic acid A was 0.6 μg/L, and ganoderic acid A had a substantial dose-response relationship in the content range of 0.9–72.9 μg/L (R2 = 0.994).
This method was used to measure the ganoderic A content of 12 varieties of G.
lucidum in the market and showed the obvious differences in the ganoderic acid A contents of the different varieties.
This method is simple, fast, and of great importance to the quality control of Ganoderma products.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Shengyun& Yuan, Yaowu& Yu, Chenchen& Gao, Hao& Tan, Jianxin& Tian, Yiling. 2020. Establishment and Application of a Method for the Determination of Ganoderic Acid A. Journal of Food Quality،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1184733
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Shengyun…[et al.]. Establishment and Application of a Method for the Determination of Ganoderic Acid A. Journal of Food Quality No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1184733
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Shengyun& Yuan, Yaowu& Yu, Chenchen& Gao, Hao& Tan, Jianxin& Tian, Yiling. Establishment and Application of a Method for the Determination of Ganoderic Acid A. Journal of Food Quality. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1184733
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1184733
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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