Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activator Using Human-Induced Pluripotent Stem Cells
المؤلفون المشاركون
Miki, Atsushi
Kiryu, Junichi
Kamao, Hiroyuki
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-19
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Purpose.
The evaluation of drug-induced cytotoxicity is of great importance for the clinical application of pharmaceutical products, and human-induced pluripotent stem cells (hiPSCs) have received considerable scrutiny as a cell source for in vitro cytotoxicity testing.
The aim of this study is to validate the concept of cytotoxicity testing using hiPSC-derived retinal pigment epithelium (hiPSC-RPE) by comparing the responsiveness of human fetal RPE (hfRPE) and human RPE cell line (ARPE19) to recombinant tissue plasminogen activator (rtPA).
Methods.
HfRPE, two types of hiPSC-RPE, and ARPE19 were cultured in media with or without rtPA.
A lactate dehydrogenase release assay was performed to investigate the dose- and time-dependent effects of rtPA on cell death.
RPE function was evaluated by measuring the secretion of pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) and RPE-specific gene expression.
Results.
Rates of cell damage in hfRPE and both hiPS-RPE were increased by rtPA supplementation (2000 and 4000 μg/ml) for 1 hour, whereas ARPE19 cell damage was increased by supplementation with rtPA at concentrations higher than 50 μg/ml.
Although 100 μg/ml rtPA for 24 hours did not affect RPE cell function, sustained rtPA exposure induced prolonged cytotoxic effects in hfRPE and two hiPSC-RPE, but not ARPE19.
Conclusion.
The responsiveness of hiPSC-RPE to rtPA is similar to that of hfRPE in terms of cell death and cell function.
Thus, hiPSC-RPE is a valuable cell source for in vitro cytotoxicity testing.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kamao, Hiroyuki& Miki, Atsushi& Kiryu, Junichi. 2019. Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activator Using Human-Induced Pluripotent Stem Cells. Journal of Ophthalmology،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1186070
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kamao, Hiroyuki…[et al.]. Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activator Using Human-Induced Pluripotent Stem Cells. Journal of Ophthalmology No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1186070
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kamao, Hiroyuki& Miki, Atsushi& Kiryu, Junichi. Evaluation of Retinal Pigment Epithelial Cell Cytotoxicity of Recombinant Tissue Plasminogen Activator Using Human-Induced Pluripotent Stem Cells. Journal of Ophthalmology. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1186070
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1186070
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر