![](/images/graphics-bg.png)
Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis
المؤلفون المشاركون
Adewumi, Moses Olubusuyi
Faleye, Temitope Oluwasegun Cephas
Adeniji, Johnson Adekunle
Ayeni, F. A.
Ibrahim, A.
Tijani, K. A.
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-28
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
This study was designed to compare both the cell culture dependent and independent enterovirus detection algorithms recommended by the WHO and assess how either might impact our perception of the diversity of enterovirus types present in a sample.
Sixteen paired samples (16 isolates from RD cell culture and their corresponding stool suspension, i.e., 32 samples) from AFP cases in Nigeria were analyzed in this study.
All the samples were subjected to RNA extraction, cDNA synthesis, the WHO recommended RT-snPCR, and its modification.
Amplicons were sequenced and strains identified.
Enterovirus diversity was the same between the isolates and fecal suspension for the control and five of the samples.
It was, however, different for the remaining 10 (62.5%) samples.
Nine (CV-B4, E6, E7, E13, E14, E19, E29, EV-B75, and EV-B77) and five (CV-A1, CV-A11, CV-A13, EV-C99, and PV2) EV-B and EV-C types, respectively, were detected.
Particularly, E19 and EV-B75 were only recovered from the isolates while E14, EV-B77, CV-A11, and CV-A13 were only recovered from fecal suspension.
Both the cell culture dependent and independent protocols bias our perception of the diversity of enterovirus types present in a sample.
Hence, effort should be directed at harmonizing both for increased sensitivity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Adeniji, Johnson Adekunle& Ayeni, F. A.& Ibrahim, A.& Tijani, K. A.& Faleye, Temitope Oluwasegun Cephas& Adewumi, Moses Olubusuyi. 2017. Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis. Journal of Pathogens،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1186145
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Adeniji, Johnson Adekunle…[et al.]. Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis. Journal of Pathogens No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1186145
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Adeniji, Johnson Adekunle& Ayeni, F. A.& Ibrahim, A.& Tijani, K. A.& Faleye, Temitope Oluwasegun Cephas& Adewumi, Moses Olubusuyi. Comparison of Algorithms for the Detection of Enteroviruses in Stool Specimens from Children Diagnosed with Acute Flaccid Paralysis. Journal of Pathogens. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1186145
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1186145
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)