Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage
المؤلفون المشاركون
Zheng, Ying
Zhou, Zhi-Guang
Luo, Xi
Qin, Zailong
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
A macrophage is an important component of innate immunity which can be activated by infection.
A series of inflammatory cytokines are produced and released to eliminate pathogens.
CpG DNA is an immune stimulator recognized by TLR9, subsequently inducing inflammatory responses in macrophages.
Long noncoding RNA (lncRNA) is a novel class of noncoding RNA, whose length is more than 200 nt, but without protein-coding capacity.
lncRNAs are involved in many physiological and pathological processes, including inflammatory responses.
In our study, a lncRNA microarray assay was performed to identify differentially expressed lncRNAs and mRNAs in RAW264.7 cells at different time points following CpG ODN stimulation.
The results revealed that expression levels of 734 lncRNAs and 734 mRNAs were altered at all time points.
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses were performed to predict the functions of dysregulated genes.
Coexpression networks of lncRNA-mRNA were constructed based on the correlation analysis between differentially expressed lncRNAs and 10 selected upregulated mRNAs, which have been reported to be involved in CpG DNA-induced inflammatory responses.
In addition, we selected 8 dysregulated lncRNAs for further validation by quantitative real-time PCR.
The present study provided a systematic perspective on the potential functions of lncRNAs in CpG ODN-induced macrophage activation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zheng, Ying& Luo, Xi& Qin, Zailong& Zhou, Zhi-Guang. 2020. Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1186893
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zheng, Ying…[et al.]. Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage. Journal of Immunology Research No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1186893
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zheng, Ying& Luo, Xi& Qin, Zailong& Zhou, Zhi-Guang. Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1186893
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1186893
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر