Perivascular Lymphocyte Clusters Induced by Gastric Subserous Layer Vaccination Mediate Optimal Immunity against Helicobacter through Facilitating Immune Cell Infiltration and Local Antibody Response
المؤلفون المشاركون
Hu, Chupeng
Liu, Wei
Xu, Ningyin
Huang, An
Zeng, Zhiqin
Luo, Shuanghui
Zhang, Zhenxing
Fan, Menghui
Ye, Feng
Xi, Tao
Xing, Yingying
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-11
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
In situ vaccination-induced local inflammatory response resulted in the establishment of a pool of tissue-resident memory T (TRM) cells and new vessels after the resolution of inflammation.
TRM cells have received increasing attention; however, the role of new vessels in protective response is still unknown.
Materials and Methods.
We performed the laparotomy to access the stomach and injected alum-based vaccine into the gastric subserous layer (GSL).
At 28 days post vaccination, a parabiosis mouse model along with depletion of anti-CD90.2 antibody was employed to explore the function of perivascular lymphocyte clusters in recall responses.
The composition of the gastric lymphocyte clusters was analyzed by immunofluorescence staining.
Antibody responses were detected using ELISA.
Gastric lymphocytes were analyzed using flow cytometry.
Results.
GSL vaccination induced the formation of new vessels in the inflamed region.
These new vessels were different from native vessels in that they were generally accompanied by perivascular lymphocyte clusters that mainly consisted of CD90-expressing cells.
Additionally, histological analysis revealed the presence of CD4+ and CD8+ T cells in the perivascular lymphocyte clusters.
Administration of a dose of an anti-CD90.2 antibody to GSL-vaccinated mice resolved these clusters.
The efficacy of protection was compared in the parabiosis mice.
Upon challenge, the presence of perivascular lymphocyte clusters was responsible for the fast recall response, as depletion of these clusters by CD90.2 antibody administration resulted in decreased expressions of VCAM-1, Madcam-1, and TNF-α, as well as lower recruitment of proinflammatory immune cells, decreased antibody levels, and poor protection.
Conclusions.
Our research demonstrates that in situ vaccination-induced regional inflammatory response contributes to optimal recall response not only by establishing a CD4+ TRM pool but also by creating an “expressway,” i.e., perivascular lymphocyte cluster.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hu, Chupeng& Liu, Wei& Xu, Ningyin& Huang, An& Zeng, Zhiqin& Luo, Shuanghui…[et al.]. 2020. Perivascular Lymphocyte Clusters Induced by Gastric Subserous Layer Vaccination Mediate Optimal Immunity against Helicobacter through Facilitating Immune Cell Infiltration and Local Antibody Response. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1186905
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hu, Chupeng…[et al.]. Perivascular Lymphocyte Clusters Induced by Gastric Subserous Layer Vaccination Mediate Optimal Immunity against Helicobacter through Facilitating Immune Cell Infiltration and Local Antibody Response. Journal of Immunology Research No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1186905
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hu, Chupeng& Liu, Wei& Xu, Ningyin& Huang, An& Zeng, Zhiqin& Luo, Shuanghui…[et al.]. Perivascular Lymphocyte Clusters Induced by Gastric Subserous Layer Vaccination Mediate Optimal Immunity against Helicobacter through Facilitating Immune Cell Infiltration and Local Antibody Response. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1186905
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1186905
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر