CpG-ODN Induces a Dose-Dependent Enrichment of Immunological Niches in the Spleen and Lungs of Neonatal Chicks That Correlates with the Protective Immunity against Escherichia coli

المؤلفون المشاركون

Tikoo, Suresh K.
Gunawardana, Thushari
Ahmed, Khawaja Ashfaque
Goonewardene, Kalhari
Popowich, Shelly
Kurukulasuriya, Shanika
Karunarathana, Ruwani
Ayalew, Lisanework E.
Gupta, Ashish
Lockerbie, Betty
Foldvari, Marianna
Willson, Philip
Gomis, Susantha

المصدر

Journal of Immunology Research

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-15، 15ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-01-13

دولة النشر

مصر

عدد الصفحات

15

التخصصات الرئيسية

الأحياء

الملخص EN

Immunoprotective function of oligodeoxynucleotides containing CpG motifs (CpG-ODN) has been demonstrated in neonatal chickens against common bacterial pathogens such as E.coli and Salmonella sp.

Our recent study reported that CpG-ODN administration enriches immune compartments in neonatal chicks.

However, a causal relationship between CpG-ODN-induced immune enrichment and protective mechanisms remains unestablished.

In this study, we investigated in ovo administered CpG-ODN-mediated immune cell recruitment in the immunological niches in lymphoid (spleen) and nonlymphoid (lungs) organs using various doses of CpG-ODN and examined whether the immunological profiles have any correlation with immunoprotection against E.coli infection.

Eighteen-day-old embryonated eggs were injected with either 5, 10, 25, and 50 μg of CpG-ODN or saline (n=~40 per group).

On the day of hatch (72 hr after CpG-ODN treatment), we collected the spleen and lungs (n=3‐4 per group) and examined the recruitment of macrophages/monocytes, their expression of MHCII and CD40, and the number of CD4+ and CD8+ T-cell subsets in the immunological niches in the spleen and lungs using flow cytometry.

We observed the dose-dependent recruitment of immune cells, wherein 25 μg and 50 μg of CpG-ODN induced significant enrichment of immunological niches in both the spleen and the lungs.

Four days after the CpG-ODN treatment (1-day after hatch), chicks were challenged with a virulent strain of E.

coli (1×104 or 1×105 cfu, subcutaneously).

Clinical outcome and mortality were monitored for 8 days postchallenge.

We found that both 25 μg and 50 μg of CpG-ODN provided significant protection and reduced clinical scores compared to saline controls against E.

coli infection.

Overall, the present study revealed that CpG-ODNs orchestrate immunological niches in neonatal chickens in a dose-dependent manner that resulted in differential protection against E.

coli infection, thus supporting a cause and effect relationship between CpG-ODN-induced immune enrichment and the antibacterial immunity.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Gunawardana, Thushari& Ahmed, Khawaja Ashfaque& Goonewardene, Kalhari& Popowich, Shelly& Kurukulasuriya, Shanika& Karunarathana, Ruwani…[et al.]. 2020. CpG-ODN Induces a Dose-Dependent Enrichment of Immunological Niches in the Spleen and Lungs of Neonatal Chicks That Correlates with the Protective Immunity against Escherichia coli. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1187065

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Gunawardana, Thushari…[et al.]. CpG-ODN Induces a Dose-Dependent Enrichment of Immunological Niches in the Spleen and Lungs of Neonatal Chicks That Correlates with the Protective Immunity against Escherichia coli. Journal of Immunology Research No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1187065

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Gunawardana, Thushari& Ahmed, Khawaja Ashfaque& Goonewardene, Kalhari& Popowich, Shelly& Kurukulasuriya, Shanika& Karunarathana, Ruwani…[et al.]. CpG-ODN Induces a Dose-Dependent Enrichment of Immunological Niches in the Spleen and Lungs of Neonatal Chicks That Correlates with the Protective Immunity against Escherichia coli. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1187065

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1187065