ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells
المؤلفون المشاركون
Guo, Xingjing
Chen, Dan
An, Shuhong
Wang, Zhaojin
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-04
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Calcitonin gene-related peptide (CGRP) is a mediator of microglial activation at the transcriptional level.
The involvement of the epigenetic mechanism in this process is largely undefined.
Histone deacetylase (HDAC)1/2 are considered important epigenetic regulators of gene expression in activated microglia.
In this study, we examined the effect of CGRP on HDAC2-mediated gene transcription in microglial cells through the chromatin immunoprecipitation sequencing (ChIP-seq) method.
Immunofluorescence analysis showed that mouse microglial cells (BV2) expressed CGRP receptor components.
Treatment of microglia with CGRP increased HDAC2 protein expression.
ChIP-seq data indicated that CGRP remarkably altered promoter enrichments of HDAC2 in microglial cells.
We identified 1271 gene promoters, whose HDAC2 enrichments are significantly altered in microglia after CGRP treatment, including 1181 upregulating genes and 90 downregulating genes.
Bioinformatics analyses showed that HDAC2-enriched genes were mainly associated with immune- and inflammation-related pathways, such as nitric oxide synthase (NOS) biosynthetic process, retinoic acid-inducible gene- (RIG-) like receptor signaling pathway, and nuclear factor kappa B (NF-κB) signaling pathway.
The expression of these key pathways (NOS, RIG-I, and NF-κB) were further verified by Western blot.
Taken together, our findings suggest that genes with differential HDAC2 enrichments induced by CGRP function in diverse cellular pathways and many are involved in immune and inflammatory responses.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guo, Xingjing& Chen, Dan& An, Shuhong& Wang, Zhaojin. 2020. ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1187194
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guo, Xingjing…[et al.]. ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells. Journal of Immunology Research No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1187194
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guo, Xingjing& Chen, Dan& An, Shuhong& Wang, Zhaojin. ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1187194
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1187194
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر