Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP Pathway
المؤلفون المشاركون
Liu, Jia-Huang
Wu, Qi-Fei
Fu, Jun-Ke
Che, Xiang-Ming
Li, Hai-Jun
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-11
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Obesity could increase the risk of esophageal squamous cell carcinoma (ESCC) and affect its growth and progression, but the mechanical links are unclear.
The objective of the study was to explore the impact of obesity on ESCC growth and progression utilizing in vivo trials and cell experiments in vitro.
Diet-induced obese and lean nude mice were inoculated with TE-1 cells, then studied for 4 weeks.
Serum glucose, insulin, leptin, and visfatin levels were assayed.
Sera of nude mice were obtained and then utilized to culture TE-1.
MTT, migration and invasion assays, RT-PCR, and Western blotting were used to analyze endocrine effect of obesity on cell proliferation, migration, invasion, and related genes expression of TE-1.
Obese nude mice bore larger tumor xenografts than lean animals, and were hyperglycemic and hyperinsulinemic with an elevated level of leptin and visfatin in sera, and also were accompanied by a fatty liver.
As for the subcutaneous tumor xenograft model, tumors were more aggressive in obese nude mice than lean animals.
Tumor weight correlated positively with mouse body weight, liver weight of mice, serum glucose, HOMA-IR, leptin, and visfatin.
Obesity prompted significant TE-1 cell proliferation, migration, and invasion by endocrine mechanisms and impacted target genes.
The expression of AMPK and p-AMPK protein decreased significantly (P<0.05); MMP9, total YAP, p-YAP, and nonphosphorylated YAP protein increased significantly (P<0.05) in the cells cultured with conditioned media and xenograft tumor from the obese group; the mRNA expression of AMPK decreased significantly (P<0.05); YAP and MMP9 mRNA expression increased significantly (P<0.05) in the cells exposed to conditioned media from the obese group.
In conclusion, the altered adipokine milieu and metabolites in the context of obesity may promote ESCC growth in vivo; affect proliferation, migration, and invasion of ESCC cells in vitro; and regulate MMP9 and AMPK-YAP signaling pathway through complex effects including the endocrine effect.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Jia-Huang& Wu, Qi-Fei& Fu, Jun-Ke& Che, Xiang-Ming& Li, Hai-Jun. 2020. Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP Pathway. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1187399
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Jia-Huang…[et al.]. Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP Pathway. Journal of Immunology Research No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1187399
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Jia-Huang& Wu, Qi-Fei& Fu, Jun-Ke& Che, Xiang-Ming& Li, Hai-Jun. Obesity Potentiates Esophageal Squamous Cell Carcinoma Growth and Invasion by AMPK-YAP Pathway. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1187399
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1187399
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر