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Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1M2 Differentiation
المؤلفون المشاركون
Liao, Hui
Zhao, Xiaoyun
Li, Rongshan
Li, Yuanping
Zhang, Xilan
Zheng, Dan
Shen, Dayue
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-27
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Objective.
It has been shown that podocyte injury represents an important pathological basis that contributes to proteinuria and eventually leads to kidney failure.
High glucose (HG) activates macrophage polarization, further exacerbating HG-induced podocyte injury.
Our previous study on diabetic nephropathy rats indicated that thalidomide (Tha) has renoprotective properties.
The present study explored the effects of Tha on mRNA and protein expressions of inducible nitric oxide synthase (iNOS), tumor necrosis factor- (TNF-) α, mannose receptor (CD206), and arginase- (Arg-) 1 in HG-activated macrophages.
iNOS and TNF-α are established as markers of classically activated macrophage (M1).
CD206 and Arg-1 are regarded as markers of alternatively activated macrophages (M2).
During the experiment, the supernatants of (HG)-treated and (Tha)-treated macrophages, designated as (HG) MS and (Tha) MS, were simultaneously collected and processed.
TNF-α and interleukin- (IL-) 1β levels as well as protein expressions of nephrin and podocin in HG, (HG) MS, and (Tha) MS-cultured podocytes were evaluated.
The results showed that compared to the 11.1 mM normal glucose (NG), the 33.3 mM HG-cultured RAW 264.7 cells exhibited upregulated iNOS and TNF-α mRNAs and protein expressions, and downregulated CD206 and Arg-1 expressions significantly (p<0.05).
Tha 200 μg/ml suppressed iNOS and TNF-α, and promoted CD206 and Arg-1 expressions significantly compared to the HG group (p<0.05).
Furthermore, (HG) MS-treated podocytes showed an increase in TNF-α and IL-1β levels and a downregulation in nephrin and podocin expression significantly compared to NG-treated and HG-treated podocytes (p<0.05).
The (Tha 200 μg/ml) MS group exhibited a decrease in TNF-α and IL-1β level, and an upregulation in nephrin and podocin expressions significantly compared to the (HG) MS group (p<0.05).
Our research confirmed that HG-activated macrophage differentiation aggravates HG-induced podocyte injury in vitro and the protective effects of Tha might be related to its actions on TNF-α and IL-1β levels via its modulation on M1/M2 differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liao, Hui& Li, Yuanping& Zhang, Xilan& Zhao, Xiaoyun& Zheng, Dan& Shen, Dayue…[et al.]. 2020. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1M2 Differentiation. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1187551
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liao, Hui…[et al.]. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1M2 Differentiation. Journal of Immunology Research No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1187551
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liao, Hui& Li, Yuanping& Zhang, Xilan& Zhao, Xiaoyun& Zheng, Dan& Shen, Dayue…[et al.]. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1M2 Differentiation. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1187551
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1187551
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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