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Electric Cell-Substrate Impedance Sensing of Cellular Effects under Hypoxic Conditions and Carbonic Anhydrase Inhibition
المؤلفون المشاركون
Stan, Miruna Silvia
Stanica, Luciana
Rosu-Hamzescu, Mihnea
Gheorghiu, Mihaela
Antonescu, Loredana
Polonschii, Cristina
Gheorghiu, Eugen
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-27
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Tumor hypoxia provides a dynamic environment for the cancer cells to thrive and metastasize.
Evaluation of cell growth, cell-cell, and cell surface interactions in hypoxic conditions is therefore highly needed in the establishment of treatment options.
Electric cell-substrate impedance sensing (ECIS) has been traditionally used in the evaluation of cellular platforms as a real-time, label-free impedance-based method to study the activities of cells grown in tissue cultures, but its application for hypoxic environments is seldom reported.
We present real-time evaluation of hypoxia-induced bioeffects with a focus on hypoxic pH regulation of tumor environment.
To this end, multiparametric real-time bioanalytical platform using electrical impedance spectroscopy (EIS) and human colon cancer HT-29 cells is advanced.
A time series of EIS data enables monitoring with high temporal resolution the alterations occurring within the cell layer, especially at the cell-substrate level.
We reveal the dynamic changes of cellular processes during hypoxic conditions and in response to application of acetazolamide (AZA), a carbonic anhydrase inhibitor.
Optical evaluation and pH assessment complemented the electrical analysis towards establishing a pattern of cellular changes.
The proposed bioanalytical platform indicates wide applicability towards evaluation of bioeffects of hypoxia at cellular level.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Stanica, Luciana& Rosu-Hamzescu, Mihnea& Gheorghiu, Mihaela& Stan, Miruna Silvia& Antonescu, Loredana& Polonschii, Cristina…[et al.]. 2017. Electric Cell-Substrate Impedance Sensing of Cellular Effects under Hypoxic Conditions and Carbonic Anhydrase Inhibition. Journal of Sensors،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1187654
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Stanica, Luciana…[et al.]. Electric Cell-Substrate Impedance Sensing of Cellular Effects under Hypoxic Conditions and Carbonic Anhydrase Inhibition. Journal of Sensors No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1187654
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Stanica, Luciana& Rosu-Hamzescu, Mihnea& Gheorghiu, Mihaela& Stan, Miruna Silvia& Antonescu, Loredana& Polonschii, Cristina…[et al.]. Electric Cell-Substrate Impedance Sensing of Cellular Effects under Hypoxic Conditions and Carbonic Anhydrase Inhibition. Journal of Sensors. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1187654
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1187654
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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