Identification and Functional Analysis of EPOR+ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis
المؤلفون المشاركون
Li, Yanxing
Li, Ming
Wei, Rong
Wu, Junlong
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-18
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Background.
Tissue-resident macrophages can be educated to tumor-associated macrophages (TAMs) by the tumor microenvironment and many types of macrophages express erythropoietic receptor (EPOR); However, little is known about the expression of EPOR on TAMs and the identity of EPOR+ TAMs in osteosarcoma lung metastasis has thus far remained elusive.
Methods.
EPOR-eGFPcre mice were used to determine the expression of EPOR on lung tissue-resident macrophages.
Flow cytometry, RT-PCR, and Western blot were examined to define the identity of EPOR+ TAMs in 106 osteosarcoma lung metastasis specimens.
Moreover, the clinicopathologic factors and prognosis of patients with CD163+EPOR+ macrophages were compared.
Results.
We found that a subpopulation of mouse lung tissue-resident macrophages express EPOR and EPO enhances the proliferation of EPOR+ macrophages in mouse lung.
A subpopulation of CD163+ macrophages expresses EPOR in human osteosarcoma lung metastasis specimens.
CD163+EPOR+TAMs increase 2.5 times in human osteosarcoma lung metastasis tissues; CD206, CD163, and PD1, which are known to have a significant role in TAM function had high expression in CD163+EPOR+ TAMs compared with CD163+EPOR- TAMs.
Furthermore, CD163+EPOR+ TAMs had higher M2 marker and cytokine expression in osteosarcoma tissues compared with para-osteosarcoma tissues.
EPO enhanced the expression of M2 cytokines in primary CD163+EPOR+ TAMs.
Importantly, the percentage of CD163+EPOR+ TAMs had a positive linear association with malignant phenotypes as well as poor disease-free survival and overall survival time.
Conclusions.
We have characterized TAMs expressing EPOR and CD163+EPOR+ macrophages as TAMs in osteosarcoma lung metastasis patients, which are highly associated with tumor aggressiveness.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Yanxing& Li, Ming& Wei, Rong& Wu, Junlong. 2020. Identification and Functional Analysis of EPOR+ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1187680
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Yanxing…[et al.]. Identification and Functional Analysis of EPOR+ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis. Journal of Immunology Research No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1187680
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Yanxing& Li, Ming& Wei, Rong& Wu, Junlong. Identification and Functional Analysis of EPOR+ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1187680
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1187680
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر