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A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae
المؤلفون المشاركون
Yean Yean, Chan
Khazani, Nur Amalina
Noor, Nik Zuraina Nik Mohd
Hasan, Habsah
Suraiya, Siti
Mohamad, Suharni
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Klebsiella pneumoniae and Haemophilus influenzae are two common pathogens associated with respiratory tract infections.
The identification of these pathogens using conventional molecular diagnostic tests requires trained personnel, cold-chain transportation, and storage-dependance, which does not render them user-friendly.
The aim of this study was to develop a thermostabilized, cold-chain-free, one-step multiplex PCR for simultaneous detection of K.
pneumoniae and H.
influenzae.
The multiplex PCR assay was designed to amplify the php gene of K.
pneumoniae (202 bp) and p6 gene of H.
influenzae (582 bp).
In addition, the specific primer to amplify glm gene of Helicobacter pylori (105 bp) was included as an internal amplification control.
Subsequently, the designed primers and all PCR reagents were thermostabilized by lyophilization.
The stability of the thermostabilized PCR was evaluated using the Q10 method.
The sensitivity and specificity of performances for thermostabilized PCR were evaluated using 127 clinical isolates and were found to be 100% sensitive and specific.
The thermostabilized PCR mix was found to be stable for 30 days and the Q10 accelerated stability was found to be 3.02 months.
A cold-chain-free, PCR assay for easy, rapid, and simultaneous detection of K.
pneumoniae and H.
influenzae was successfully developed in this study.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khazani, Nur Amalina& Noor, Nik Zuraina Nik Mohd& Yean Yean, Chan& Hasan, Habsah& Suraiya, Siti& Mohamad, Suharni. 2017. A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae. Journal of Tropical Medicine،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1187945
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khazani, Nur Amalina…[et al.]. A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae. Journal of Tropical Medicine No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1187945
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khazani, Nur Amalina& Noor, Nik Zuraina Nik Mohd& Yean Yean, Chan& Hasan, Habsah& Suraiya, Siti& Mohamad, Suharni. A Thermostabilized, One-Step PCR Assay for Simultaneous Detection of Klebsiella pneumoniae and Haemophilus influenzae. Journal of Tropical Medicine. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1187945
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1187945
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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