Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania)‎ infantum

المؤلفون المشاركون

Medeiros, Fernanda Alvarenga Cardoso
Gomes, Luciana Inácia
Oliveira, Edward
de Souza, Carolina Senra Alves
Mourão, Maria Vitória
Cota, Gláucia Fernandes
Marques, Letícia Helena dos Santos
Carneiro, Mariângela
Rabello, Ana

المصدر

Journal of Tropical Medicine

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-01-09

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

A kDNA PCR enzyme-linked immunosorbent assay (kDNA PCR-ELISA) for the diagnosis of human visceral leishmaniasis (HVL) was developed.

The detection limit of the reaction, precision measurements, and cut-off of the kDNA PCR-ELISA were defined in a proof-of-concept phase.

A reference strain of Leishmania (Leishmania) infantum and a bank of 14 peripheral blood samples from immunocompetent patients with VL were characterized using techniques considered gold standards, and 11 blood samples obtained from healthy individuals of an endemic area were also assessed.

Phase II evaluation determined the performance of the assay in peripheral blood samples from 105 patients with VL (adults and children), 25 patients with Leishmania/HIV coinfection, 40 healthy individuals, and 33 asymptomatic individuals living in endemic areas.

The kDNA PCR-ELISA exhibited satisfactory precision, with a detection limit of 0.07 fg of DNA from L.

(L.) infantum and 1 parasite/mL blood.

The overall sensitivity of the assay for all groups studied was 100% (95% confidence interval [CI]: 97.1–100%), and the specificity was 95% (95% CI: 83.5–98.6%).

The kDNA PCR-ELISA was shown to be a useful tool for VL symptomatic and asymptomatic individuals diagnosis and its use in endemic countries may help monitor control interventions.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Medeiros, Fernanda Alvarenga Cardoso& Gomes, Luciana Inácia& Oliveira, Edward& de Souza, Carolina Senra Alves& Mourão, Maria Vitória& Cota, Gláucia Fernandes…[et al.]. 2017. Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum. Journal of Tropical Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1187946

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Medeiros, Fernanda Alvarenga Cardoso…[et al.]. Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum. Journal of Tropical Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1187946

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Medeiros, Fernanda Alvarenga Cardoso& Gomes, Luciana Inácia& Oliveira, Edward& de Souza, Carolina Senra Alves& Mourão, Maria Vitória& Cota, Gláucia Fernandes…[et al.]. Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum. Journal of Tropical Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1187946

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1187946