Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation
المؤلفون المشاركون
Vaiyapuri, Sakthivel
Zeuner, Marie-Theres
Vallance, Thomas
Cottrell, Graeme S.
Widera, Darius
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-16
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Aberrant activation of the transcription factor NF-κB, as well as uncontrolled inflammation, has been linked to autoimmune diseases, development and progression of cancer, and neurological disorders like Alzheimer’s disease.
Reporter cell lines are a valuable state-of-the art tool for comparative analysis of in vitro drug screening.
However, a reporter cell line for the investigation of NF-κB-driven neuroinflammation has not been available.
Thus, we developed a stable neural NF-κB-reporter cell line to assess the potency of proinflammatory molecules and peptides, as well as anti-inflammatory pharmaceuticals.
We used lentivirus to transduce the glioma cell line U251-MG with a tandem NF-κB reporter construct containing GFP and firefly luciferase allowing an assessment of NF-κB activity via fluorescence microscopy, flow cytometry, and luminometry.
We observed a robust activation of NF-κB after exposure of the reporter cell line to tumour necrosis factor alpha (TNFα) and amyloid-β peptide [1-42] as well as to LPS derived from Salmonella minnesota and Escherichia coli.
Finally, we demonstrate that the U251-NF-κB-GFP-Luc reporter cells can be used for assessing the anti-inflammatory potential of pharmaceutical compounds using Bay11-7082 and IMD0354.
In summary, our newly generated cell line is a robust and cost-efficient tool to study pro- and anti-inflammatory potential of drugs and biologics in neural cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zeuner, Marie-Theres& Vallance, Thomas& Vaiyapuri, Sakthivel& Cottrell, Graeme S.& Widera, Darius. 2017. Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1188531
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zeuner, Marie-Theres…[et al.]. Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation. Mediators of Inflammation No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1188531
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zeuner, Marie-Theres& Vallance, Thomas& Vaiyapuri, Sakthivel& Cottrell, Graeme S.& Widera, Darius. Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1188531
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1188531
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر