HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the InflammatoryRepair Response of Human Keratinocytes
المؤلفون المشاركون
Calvieri, Stefano
Corsetto, Paola Antonia
Fumagalli, Marco
Colombo, Irma
Maggio, Roberta
Zava, Stefania
Corbett, Yolanda
Carlino, Claudia
Scaccabarozzi, Diletta
Mattozzi, Carlo
Taramelli, Donatella
Dell'Agli, Mario
Sangiovanni, Enrico
Gismondi, Angela
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-17
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Cultured primary human keratinocytes are frequently employed for studies of immunological and inflammatory responses; however, interpretation of experimental data may be complicated by donor to donor variability, the relatively short culture lifetime, and variations between passages.
To standardize the in vitro studies on keratinocytes, we investigated the use of HaCaT cells, a long-lived, spontaneously immortalized human keratinocyte line which is able to differentiate in vitro, as a suitable model to follow the release of inflammatory and repair mediators in response to TNFα or IL-1β.
Different treatment conditions (presence or absence of serum) and differentiation stimuli (increase in cell density as a function of time in culture and elevation of extracellular calcium) were considered.
ELISA and Multiplex measurement technologies were used to monitor the production of cytokines and chemokines.
Taken together, the results highlight that Ca2+ concentration in the medium, cell density, and presence of serum influences at different levels the release of proinflammatory mediators by HaCaT cells.
Moreover, HaCaT cells maintained in low Ca2+ medium and 80% confluent are similar to normal keratinocytes in terms of cytokine production suggesting that HaCaT cells may be a useful model to investigate anti-inflammatory interventions/therapies on skin diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Colombo, Irma& Sangiovanni, Enrico& Maggio, Roberta& Mattozzi, Carlo& Zava, Stefania& Corbett, Yolanda…[et al.]. 2017. HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the InflammatoryRepair Response of Human Keratinocytes. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1188657
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Colombo, Irma…[et al.]. HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the InflammatoryRepair Response of Human Keratinocytes. Mediators of Inflammation No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1188657
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Colombo, Irma& Sangiovanni, Enrico& Maggio, Roberta& Mattozzi, Carlo& Zava, Stefania& Corbett, Yolanda…[et al.]. HaCaT Cells as a Reliable In Vitro Differentiation Model to Dissect the InflammatoryRepair Response of Human Keratinocytes. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1188657
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1188657
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر