Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1
المؤلفون المشاركون
Wang, Yi-Zhen
Gan, Zhen-Shun
Wang, Qian-Qian
Li, Jia-Hui
Wang, Xu-Liang
Du, Hua-Hua
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-02-13
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Iron metabolism in inflammation has been mostly characterized in macrophages exposed to pathogens or inflammatory conditions.
The aim of this study is to investigate the cross-regulatory interactions between M1 macrophage polarization and iron metabolism.
Firstly, we characterized the transcription of genes related to iron homeostasis in M1 RAW264.7 macrophages stimulated by IFN-γ.
The molecular signature of M1 macrophages showed high levels of iron storage (ferritin), a low level of iron export (ferroportin), and changes of iron regulators (hepcidin and transferrin receptors), which favour iron sequestration in the reticuloendothelial system and are benefit for inflammatory disorders.
Then, we evaluated the effect of iron on M1 macrophage polarization.
Iron significantly reduced mRNA levels of IL-6, IL-1β, TNF-α, and iNOS produced by IFN-γ-polarized M1 macrophages.
Immunofluorescence analysis showed that iron also reduced iNOS production.
However, iron did not compromise but enhanced the ability of M1-polarized macrophages to phagocytose FITC-dextran.
Moreover, we demonstrated that STAT1 inhibition was required for reduction of iNOS and M1-related cytokines production by the present of iron.
Together, these findings indicated that iron decreased polarization of M1 macrophages and inhibited the production of the proinflammatory cytokines.
The results expanded our knowledge about the role of iron in macrophage polarization.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gan, Zhen-Shun& Wang, Qian-Qian& Li, Jia-Hui& Wang, Xu-Liang& Wang, Yi-Zhen& Du, Hua-Hua. 2017. Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188784
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gan, Zhen-Shun…[et al.]. Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1. Mediators of Inflammation No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1188784
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gan, Zhen-Shun& Wang, Qian-Qian& Li, Jia-Hui& Wang, Xu-Liang& Wang, Yi-Zhen& Du, Hua-Hua. Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188784
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1188784
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر