Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB

المؤلفون المشاركون

Sooranna, Suren R.
Huang, Xia
Zeng, Yi
Jiang, Yujie
Qin, Yueqiu
Luo, Weigui
Xiang, Shulin
Pinhu, Liao

المصدر

Mediators of Inflammation

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-20، 20ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-05-29

دولة النشر

مصر

عدد الصفحات

20

التخصصات الرئيسية

الأمراض

الملخص EN

Background.

LBP and fractalkine are known to be involved in the pathogenesis of ARDS.

This study investigated the relationship between LBP and fractalkine in LPS-induced A549 cells and rat lung tissue in an ARDS rat model.

Methods.

A549 cells were transfected with LBP or LBP shRNA plasmid DNA or pretreated with SB203580 or SC-514 following LPS treatment.

An ARDS rat model was established using LPS with or without LBPK95A, SB203580, or SC-514 treatment.

RT-PCR, western blotting, ELISA, immunofluorescence, coimmunoprecipitation, and immunohistochemical staining were used to study the expression of fractalkine and LBP and p38 MAPK and p65 NF-κB activities.

Results.

LPS increased LBP and reduced fractalkine.

LBP overexpression further decreased LPS-induced downregulation of fractalkine and p38 MAPK and p65 NF-κB activation; LBP gene silencing, SB203580, and SC-514 suppressed LPS-induced downregulation of fractalkine and p38 MAPK and p65 NF-κB activation in A549 cells.

LBP and fractalkine in lung tissue were increased and decreased, respectively, following LPS injection.

LBPK95A, SB203580, and SC-514 ameliorated LPS-induced rat lung injury and suppressed LPS-induced downregulation of fractalkine by decreasing phospho-p38 MAPK and p65 NF-κB.

Conclusions.

The results indicate that LBP downregulates fractalkine expression in LPS-induced A549 cells and in an ARDS rat model through activation of p38 MAPK and NF-κB.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Huang, Xia& Zeng, Yi& Jiang, Yujie& Qin, Yueqiu& Luo, Weigui& Xiang, Shulin…[et al.]. 2017. Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-20.
https://search.emarefa.net/detail/BIM-1188841

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Huang, Xia…[et al.]. Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB. Mediators of Inflammation No. 2017 (2017), pp.1-20.
https://search.emarefa.net/detail/BIM-1188841

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Huang, Xia& Zeng, Yi& Jiang, Yujie& Qin, Yueqiu& Luo, Weigui& Xiang, Shulin…[et al.]. Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-20.
https://search.emarefa.net/detail/BIM-1188841

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1188841