An Early Myeloma Bone Disease Model in Skeletally Mature Mice as a Platform for Biomaterial Characterization of the Extracellular Matrix
المؤلفون المشاركون
Ziouti, Fani
Soares, Ana Prates
Moreno-Jiménez, Inés
Rack, Alexander
Bogen, Bjarne
Cipitria, Amaia
Zaslansky, Paul
Jundt, Franziska
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-27
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Multiple myeloma (MM) bone disease is characterized by osteolytic bone tissue destruction resulting in bone pain, fractures, vertebral collapse, and spinal cord compression in patients.
Upon initial diagnosis of MM, almost 80% of patients suffer from bone disease.
Earlier diagnosis and intervention in MM bone disease would potentially improve treatment outcome and patient survival.
New preclinical models are needed for developing novel diagnostic markers of bone structural changes as early as possible in the disease course.
Here, we report a proof-of-concept, syngeneic, intrafemoral MOPC315.BM MM murine model in skeletally mature BALB/c mice for detection and characterization of very early changes in the extracellular matrix (ECM) of MM-injected animals.
Bioluminescence imaging (BLI) in vivo confirmed myeloma engraftment in 100% of the animals with high osteoclast activity within 21 days after tumor cell inoculation.
Early signs of aggressive bone turnover were observed on the outer bone surfaces by high-resolution microcomputed tomography (microCT).
Synchrotron phase contrast-enhanced microcomputer tomography (PCE-CT) revealed very local microarchitecture differences highlighting numerous active sites of erosion and new bone at the micrometer scale.
Correlative backscattered electron imaging (BSE) and confocal laser scanning microscopy allowed direct comparison of mineralized and nonmineralized matrix changes in the cortical bone.
The osteocyte lacunar-canalicular network (OLCN) architecture was disorganized, and irregular-shaped osteocyte lacunae were observed in MM-injected bones after 21 days.
Our model provides a potential platform to further evaluate pathological MM bone lesion development at the micro- and ultrastructural levels.
These promising results make it possible to combine material science and pharmacological investigations that may improve early detection and treatment of MM bone disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ziouti, Fani& Soares, Ana Prates& Moreno-Jiménez, Inés& Rack, Alexander& Bogen, Bjarne& Cipitria, Amaia…[et al.]. 2020. An Early Myeloma Bone Disease Model in Skeletally Mature Mice as a Platform for Biomaterial Characterization of the Extracellular Matrix. Journal of Oncology،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1188929
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ziouti, Fani…[et al.]. An Early Myeloma Bone Disease Model in Skeletally Mature Mice as a Platform for Biomaterial Characterization of the Extracellular Matrix. Journal of Oncology No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1188929
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ziouti, Fani& Soares, Ana Prates& Moreno-Jiménez, Inés& Rack, Alexander& Bogen, Bjarne& Cipitria, Amaia…[et al.]. An Early Myeloma Bone Disease Model in Skeletally Mature Mice as a Platform for Biomaterial Characterization of the Extracellular Matrix. Journal of Oncology. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1188929
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1188929
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر