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Correlation between FAK and EGF-Induced EMT in Colorectal Cancer Cells
المؤلفون المشاركون
Huang, Kun
Gao, Ningning
Bian, Donglin
Zhai, Qixi
Yang, Puxu
Li, Mingwei
Wang, Xuemei
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-02-17
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Epithelial-mesenchymal transition (EMT) plays an important role in the invasion and metastasis of colorectal cancer, which is mediated by FAK and EGF.
However, whether FAK participates in EMT in colorectal cancer cells through the EGF/EGFR signaling pathway remains unknown.
The aim of this study was to investigate the effector mechanisms of FAK in the process of EGF-induced EMT in colorectal cancer cells and to determine whether miR-217 is involved in this process.
Caco-2 cancer cells were routinely cultured with and without treatment with 100 ng/mL EGF, and changes in cell morphology were observed using an inverted microscope.
In addition, a transwell assay was used to detect cell migration under the condition of EGF treatment.
The expression of FAK, pFAK, E-cadherin, vimentin, and β actin was assessed by western blotting, and the expression of miR-217 was assessed using real-time PCR.
We found that EGF induced EMT in colorectal cancer cells and enhanced cell migration and invasion ability.
Moreover, FAK was involved in the EGF-induced EMT of colorectal cancer cells.
EGF upregulated the expression of E-cadherin in colorectal cancer cells by activating FAK, and miR-217 was found to participate in EGF-induced EMT in colorectal cancer cells.
Our findings indicate that EGF induces EMT in colorectal cancer cells by activating FAK, and miR-217 is involved in the EGF/FAK/E-cadherin signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Kun& Gao, Ningning& Bian, Donglin& Zhai, Qixi& Yang, Puxu& Li, Mingwei…[et al.]. 2020. Correlation between FAK and EGF-Induced EMT in Colorectal Cancer Cells. Journal of Oncology،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1188991
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Kun…[et al.]. Correlation between FAK and EGF-Induced EMT in Colorectal Cancer Cells. Journal of Oncology No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1188991
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Kun& Gao, Ningning& Bian, Donglin& Zhai, Qixi& Yang, Puxu& Li, Mingwei…[et al.]. Correlation between FAK and EGF-Induced EMT in Colorectal Cancer Cells. Journal of Oncology. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1188991
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1188991
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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