DNA-Detection Based Diagnostics for Taenia solium Cysticercosis in Porcine
المؤلفون المشاركون
Eric, Agola L.
Misinzo, Gerald
Waema, Maxwell W.
Ngowi, Helena A.
Kagira, John Maina
المصدر
Journal of Parasitology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-07
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Porcine cysticercosis is a neglected and underestimated disease caused by metacestode stage of the tapeworm, Taenia solium (T.
solium).
Pigs are the intermediate hosts of T.
solium while human are the only known definitive host.
The disease has an economic consequence because the affected farmers lose 50−100 percent of the value of pigs if they are infected.
Lack of affordable, easy to use, sensitive, and specific molecular diagnostic tools for detection of infections at the farm level hinders the control of porcine cysticercosis in endemic areas.
A number of DNA based diagnostic assays for the detection of T.
solium infections in pigs have been developed and evaluated but none is applicable at low-resource areas where this disease is an endemic.
This review focuses mainly on DNA based diagnostic methods, their sensitivity, specificity, and utilization at low-resource areas.
We summarized data from 65 studies on the current DNA-detection based diagnostic techniques for T.
solium cysticercosis in porcine, published in English between the years 2000–2018, identified through PubMed search engine.
Of the different polymerase chain reaction (PCR) assays developed for identification of T.
solium, the most sensitive (97−100%) and specific (100%) one is nested PCR.
One study utilized loop-mediated isothermal amplification (LAMP) as a diagnostic tool for the detection of T.
solium infections though its field use was never determined.
Recombinase polymerase amplification (RPA) has been evaluated as a diagnostic tool for a variety of diseases, but has never been exploited for the diagnosis of cysticercosis/taeniasis.
In conclusion, several molecular methods have been developed and evaluated in lab settings.
However, there is need to validate these methods as a diagnostic tool to diagnose porcine cysticercosis in low-resource areas.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Waema, Maxwell W.& Misinzo, Gerald& Kagira, John Maina& Eric, Agola L.& Ngowi, Helena A.. 2020. DNA-Detection Based Diagnostics for Taenia solium Cysticercosis in Porcine. Journal of Parasitology Research،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1190090
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Waema, Maxwell W.…[et al.]. DNA-Detection Based Diagnostics for Taenia solium Cysticercosis in Porcine. Journal of Parasitology Research No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1190090
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Waema, Maxwell W.& Misinzo, Gerald& Kagira, John Maina& Eric, Agola L.& Ngowi, Helena A.. DNA-Detection Based Diagnostics for Taenia solium Cysticercosis in Porcine. Journal of Parasitology Research. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1190090
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1190090
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر