Reduced Graphene OxideTEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination of Mycobacterium tuberculosis

المؤلفون المشاركون

Yusof, Nor Azah
Abdullah, Jaafar
Sulaiman, Yusran
Zaid, Mohd Hazani Mat
Noh, Mohd Fairulnizal Md
Issa, Rahizan
Wasoh, H.

المصدر

Journal of Sensors

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-01-21

دولة النشر

مصر

عدد الصفحات

11

التخصصات الرئيسية

هندسة مدنية

الملخص EN

A novel peptide nuclide acid (PNA) electrochemical biosensor based on reduced graphene oxide (NH2-rGO)/2,2,6,6-tetramethylpiperidin-1-yl)oxyl nanocrystalline cellulose (TEMPO-NCC) for the detection of Mycobacterium tuberculosis (M.

Tuberculosis) is described.

In this study, the nanohybrid films NH2-rGO/TEMPO-NCC were immobilized onto screen-printed carbon electrode (SPE) via a simple drop-coating method.

The electrochemical characterization of the designed electrode was investigated using cyclic voltammetry (CV) and impedance spectroscopy (EIS).

Meanwhile, the sensitivity and selectivity of the designed biosensor against M.

tuberculosis were measured by the differential pulse voltammetry (DPV).

The response of the PNA probe-modified (NH2-rGO)/TEMPO-NCC demonstrated that the fabricated biosensor was able to distinguish between complementary, noncomplementary, and one-base mismatch DNA sequences using methylene blue (MB) as the electrochemical indicator.

The developed electrochemical biosensor exhibited a linear calibration curve in the concentration range of 1×10−8 M to 1×10−13 M with the limit of detection of 3.14×10−14 M.

The developed electrochemical biosensor has also been tested with a polymerase chain reaction (PCR) product of M.

tuberculosis DNA which has shown successful results in distinguishing between negative and positive samples of M.

tuberculosis.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zaid, Mohd Hazani Mat& Abdullah, Jaafar& Yusof, Nor Azah& Wasoh, H.& Sulaiman, Yusran& Noh, Mohd Fairulnizal Md…[et al.]. 2020. Reduced Graphene OxideTEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination of Mycobacterium tuberculosis. Journal of Sensors،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1190428

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zaid, Mohd Hazani Mat…[et al.]. Reduced Graphene OxideTEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination of Mycobacterium tuberculosis. Journal of Sensors No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1190428

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zaid, Mohd Hazani Mat& Abdullah, Jaafar& Yusof, Nor Azah& Wasoh, H.& Sulaiman, Yusran& Noh, Mohd Fairulnizal Md…[et al.]. Reduced Graphene OxideTEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination of Mycobacterium tuberculosis. Journal of Sensors. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1190428

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1190428