Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes
المؤلفون المشاركون
Chen, Yong
Fujuan, Qiu
Chen, Ensheng
Yu, Beijia
Zuo, Fangfang
Yuan, Yi
Zhao, Xiaofeng
Xiao, Changhong
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-26
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objectives.
To determine differences in AIM2 inflammasome expression levels between rheumatoid arthritis (RA) and osteoarthritis (OA) and to investigate the role of AIM2 in RA fibroblast-like synoviocytes (RA-FLS).
Methods.
Serum AIM2 levels among health controls (HC, n=20), OA (n=25), and RA (n =49) patients were compared via ELISA.
The different expression levels of AIM2, ASC, caspase-1, and IL-1β between RA and OA synovium were semiquantified by qRT-PCR and immunohistochemical (IHC) staining.
IHC staining was recorded by H scores, and its correlation with the ESR and CRP levels of RA patients was determined.
SiRNA AIM2 was transferred to RA-FLS and its effects on the proliferation and migration via CCK-8 assay and Transwell test, respectively.
Results.
In RA sera, the HC expressed higher level of AIM2 than OA and RA patients, and ASC, caspase-1, and IL-1β expressed higher in RA patients than HC; no significant differences were observed between sera of OA and RA patients.
However, in affected knee synovium, AIM2, ASC, caspase-1, and IL-1β were expressed higher in RA than that of OA.
Moreover, the H scores of AIM2, ASC, and IL-1β were positively correlated with the ESR and CRP levels in RA patients.
The proliferation of FLS was significantly inhibited after transferring with AIM2 siRNA to FLS.
There were no differences in apoptosis and migration assay between the si-AIM2 group and the control group.
Conclusion.
AIM2 inflammasome pathway involves in the pathogenesis of RA.
si-AIM2 inhibits the proliferation of RA-FLS, which may be a promising therapeutic strategy for the treatment of RA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Yong& Fujuan, Qiu& Chen, Ensheng& Yu, Beijia& Zuo, Fangfang& Yuan, Yi…[et al.]. 2020. Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1191518
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Yong…[et al.]. Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes. Mediators of Inflammation No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1191518
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Yong& Fujuan, Qiu& Chen, Ensheng& Yu, Beijia& Zuo, Fangfang& Yuan, Yi…[et al.]. Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1191518
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191518
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر